GTP Regulates the Interaction between MciZ and FtsZ: A Possible Role of MciZ in Bacterial Cell Division
DSpace at IIT Bombay
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Title |
GTP Regulates the Interaction between MciZ and FtsZ: A Possible Role of MciZ in Bacterial Cell Division
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Creator |
RAY, S
KUMAR, A PANDA, D |
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Subject |
C-TERMINAL TAIL
PROTEIN FTSZ ESCHERICHIA-COLI ASSEMBLY DYNAMICS BACILLUS-SUBTILIS GUANINE-NUCLEOTIDES CYTOKINESIS BINDING CYTOSKELETON PROTOFILAMENTS |
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Description |
MciZ, a peptide with 40 amino acid residues, has been shown to be expressed during bacterial sporulation, to inhibit Z-ring formation in bacteria, and to inhibit the assembly of FtsZ in vitro. Here, MciZ was found to bind to FtsZ in vitro with a dissociation constant of 0.3 +/- 0.1 mu M. Guanosine nucleotides inhibited the binding of MciZ to FtsZ; however, GTP inhibited the binding of MciZ to FtsZ more strongly than GDP. In addition, MciZ inhibited the binding of 2',3'-O-(2,4,6-trinitrocyclohexadienylidene)-GTP, a fluorescent analogue of GTP, to FtsZ. The results indicated that MciZ shares its binding site on FtsZ with GTP. Furthermore, M19I, an N-terminal 19-residue peptide (MKVHRMPKGVVLVGKAWEI) of MciZ, inhibited the assembly and GTPase activity of FtsZ in vitro. The results suggested that GTP plays an important role in the regulation of the interaction between FtsZ and MciZ and that M19I may be used as a lead peptide to design peptide inhibitors of FtsZ assembly.
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Publisher |
AMER CHEMICAL SOC
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Date |
2014-10-15T11:50:03Z
2014-10-15T11:50:03Z 2013 |
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Type |
Article
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Identifier |
BIOCHEMISTRY, 52(2)392-401
http://dx.doi.org/10.1021/bi301237m http://dspace.library.iitb.ac.in/jspui/handle/100/14830 |
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Language |
en
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