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Prediction by Promoter Logic in Bacterial Quorum Sensing

DSpace at IIT Bombay

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Title Prediction by Promoter Logic in Bacterial Quorum Sensing
 
Creator RAI, N
ANAND, R
RAMKUMAR, K
SREENIVASAN, V
DABHOLKAR, S
VENKATESH, KV
THATTAI, M
 
Subject TO-CELL COMMUNICATION
AERUGINOSA VIRULENCE GENES
GRAM-NEGATIVE BACTERIA
POSITIVE FEEDBACK
VIBRIO-FISCHERI
ESCHERICHIA-COLI
EXPRESSION
AUTOINDUCER
ELEMENTS
CIRCUIT
 
Description Quorum-sensing systems mediate chemical communication between bacterial cells, coordinating cell-density-dependent processes like biofilm formation and virulence-factor expression. In the proteobacterial LuxI/LuxR quorum sensing paradigm, a signaling molecule generated by an enzyme (LuxI) diffuses between cells and allosterically stimulates a transcriptional regulator (LuxR) to activate its cognate promoter (pR). By expressing either LuxI or LuxR in positive feedback from pR, these versatile systems can generate smooth (monostable) or abrupt (bistable) density-dependent responses to suit the ecological context. Here we combine theory and experiment to demonstrate that the promoter logic of pR - its measured activity as a function of LuxI and LuxR levels - contains all the biochemical information required to quantitatively predict the responses of such feedback loops. The interplay of promoter logic with feedback topology underlies the versatility of the LuxI/LuxR paradigm: LuxR and LuxI positive-feedback systems show dramatically different responses, while a dual positive/negative-feedback system displays synchronized oscillations. These results highlight the dual utility of promoter logic: to probe microscopic parameters and predict macroscopic phenotype.
 
Publisher PUBLIC LIBRARY SCIENCE
 
Date 2014-10-15T12:27:28Z
2014-10-15T12:27:28Z
2012
 
Type Article
 
Identifier PLOS COMPUTATIONAL BIOLOGY, 8(1)
http://dx.doi.org/10.1371/journal.pcbi.1002361
http://dspace.library.iitb.ac.in/jspui/handle/100/14903
 
Language en