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Extrusion of electroformed giant unilamellar vesicles through track-etched membranes

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Title Extrusion of electroformed giant unilamellar vesicles through track-etched membranes
 
Creator PATIL, YP
KUMBHALKAR, MD
JADHAV, S
 
Subject Phospholipid
Bilayer
Lamellarity
Lysis tension
Encapsulation efficiency
Dynamic light scattering
LIPID VESICLES
SIZE DISTRIBUTION
CAPTURED VOLUME
LIPOSOMES
POLYDISPERSITY
ENCAPSULATION
MULTILAMELLAR
SPECTROSCOPY
PRESSURE
BILAYER
 
Description Unilamellar vesicle populations having a narrow size distribution and mean radius below 100 nm are preferred for drug delivery applications. In the present work, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) was used to prepare giant unilamellar vesicles (GUVs) by electroformation and multilamellar vesicles (MLVs) by thin film hydration. Our experiments show that in contrast to MLVs, a single-pass extrusion of GUVs through track-etched polycarbonate membranes at moderate pressure differences is sufficient to produce small liposomes having low polydispersity index. Moreover, we observe that the drug encapsulating potential of extruded liposomes obtained from GUVs is significantly higher compared to liposomes prepared by extrusion of MLVs. Furthermore, our experiments carried out for varying membrane pore diameters and extrusion pressures suggest that the size of extruded liposomes is a function of the velocity of GUV suspensions in the membrane pore. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
 
Publisher ELSEVIER IRELAND LTD
 
Date 2014-10-15T13:11:42Z
2014-10-15T13:11:42Z
2012
 
Type Article
 
Identifier CHEMISTRY AND PHYSICS OF LIPIDS, 165(4)475-481
http://dx.doi.org/10.1016/j.chemphyslip.2011.11.013
http://dspace.library.iitb.ac.in/jspui/handle/100/14972
 
Language en