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Engineered-membranes: A novel concept for clustering of native lipid bilayers

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Title Engineered-membranes: A novel concept for clustering of native lipid bilayers
 
Creator PATCHORNIK, G
NAMBOOTHIRI, INN
NAIR, DK
WACHTEL, E
COHEN, SR
FRIEDMAN, N
SHEVES, M
 
Subject Lipid bilayer
Membrane protein
Specific conjugation
Hydrophobic chelator
Bathophenanthroline
TOTAL INTERNAL-REFLECTION
SUPPORTED BILAYERS
BACTERIORHODOPSIN
SPECTROSCOPY
MECHANISM
PROTEINS
FUSION
 
Description A strategy for clustering of native lipid membranes is presented. It relies on the formation of complexes between hydrophobic chelators embedded within the lipid bilayer and metal cations in the aqueous phase, capable of binding two (or more) chelators simultaneously Fig. 1. We used this approach with purple membranes containing the light driven proton pump protein bacteriorhodopsin (bR) and showed that patches of purple membranes cluster into mm sized aggregates and that these are stable for months when incubated at 19 C in the dark. The strategy may be general since four different hydrophobic chelators (1,10-phenanthroline, bathophenanthroline, Phen-Cl 0, and 8-hydroxyquinoline) and various divalent cations (Ni2+, Zn2+, Cd2+, Mn2+, and Cu2+) induced formation of membrane clusters. Moreover, the absolute requirement for a hydrophobic chelator and the appropriate metal cations was demonstrated with light and atomic force microscopy (AFM); the presence of the metal does not appear to affect the functional state of the protein. The potential utility of the approach as an alternative to assembled lipid bilayers is suggested. (C) 2012 Elsevier Inc. All rights reserved.
 
Publisher ACADEMIC PRESS INC ELSEVIER SCIENCE
 
Date 2014-10-15T13:24:39Z
2014-10-15T13:24:39Z
2012
 
Type Article
 
Identifier JOURNAL OF COLLOID AND INTERFACE SCIENCE, 388300-305
http://dx.doi.org/10.1016/j.jcis.2012.08.024
http://dspace.library.iitb.ac.in/jspui/handle/100/14997
 
Language en