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Quantification of metabolism in Saccharomyces cerevisiae under hyperosmotic conditions using elementary mode analysis

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Title Quantification of metabolism in Saccharomyces cerevisiae under hyperosmotic conditions using elementary mode analysis
 
Creator PARMAR, JH
BHARTIYA, S
VENKATESH, KV
 
Subject Yeast
Central metabolism
Hyperosmotic stress
Elementary mode analysis
Optimal feasible space
MINIMAL CUT SETS
ESCHERICHIA-COLI
PATHWAY ANALYSIS
BIOCHEMICAL NETWORKS
OSMOTIC-STRESS
YEAST
DEFINITION
ADAPTATION
GLYCEROL
BIOMASS
 
Description Yeast metabolism under hyperosmotic stress conditions was quantified using elementary mode analysis to obtain insights into the metabolic status of the cell. The fluxes of elementary modes were determined as solutions to a linear program that used the stoichiometry of the elementary modes as constraints. The analysis demonstrated that distinctly different sets of elementary modes operate under normal and hyperosmotic conditions. During the adaptation phase, elementary modes that only produce glycerol are active, while elementary modes that yield biomass, ethanol, and glycerol become active after the adaptive phase. The flux distribution in the metabolic network, calculated using the fluxes in the elementary modes, was employed to obtain the flux ratio at key nodes. At the glucose 6-phosphate (G6P) node, 25% of the carbon influx was diverted towards the pentose phosphate pathway under normal growth conditions, while only 0.3% of the carbon flux was diverted towards the pentose phosphate pathway during growth at 1 M NaCl, indicating that cell growth is arrested under hyperosmotic conditions. Further, objective functions were used in the linear program to obtain optimal solution spaces corresponding to the different accumulation rates. The analysis demonstrated that while biomass formation was optimal under normal growth conditions, glycerol synthesis was closer to optimal during adaptation to osmotic shock.
 
Publisher SPRINGER HEIDELBERG
 
Date 2014-10-15T13:26:12Z
2014-10-15T13:26:12Z
2012
 
Type Article
 
Identifier JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 39(6)927-941
http://dx.doi.org/10.1007/s10295-012-1090-4
http://dspace.library.iitb.ac.in/jspui/handle/100/15000
 
Language en