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An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA

DSpace at IIT Bombay

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Title An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA
 
Creator ONGKUDON, CM
PAN, S
DANQUAH, MK
 
Subject Monolith
Chromatography
DNA isolation
Fosmid DNA
PLASMID DNA
CHROMATOGRAPHY
FLOW
POLYMERIZATION
PURIFICATION
DEPENDENCE
DILUTE
 
Description The use of large DNAs in preparing multivalent vaccines that will eventually give protective immunity against multiple pathogenic microbes is becoming a major debate nowadays. One of the important issues in ensuring the successful implementation of the new vaccine technology is the development of a chromatographic technique that can handle larger DNAs. This paper reports the development of a novel conical monolithic column format with pore and surface characteristics engineered for the isolation of 25 kbp DNA in a single step fashion. An effective method of eliminating wall channelling, a defect of most conventional monolithic chromatography systems which has caused significant loss of product, was applied to maximise DNA recovery. This method was based on a systematic reduction of wall channel size based on a predetermined correlation between column's back pressure and wall channel size of a particular monolith pore size. (C) 2013 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2014-10-15T14:00:43Z
2014-10-15T14:00:43Z
2013
 
Type Article
 
Identifier JOURNAL OF CHROMATOGRAPHY A, 1318156-162
0021-9673
1873-3778
http://dx.doi.org/10.1016/j.chroma.2013.10.011
http://dspace.library.iitb.ac.in/jspui/handle/100/15049
 
Language en