An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA
DSpace at IIT Bombay
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Title |
An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA
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Creator |
ONGKUDON, CM
PAN, S DANQUAH, MK |
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Subject |
Monolith
Chromatography DNA isolation Fosmid DNA PLASMID DNA CHROMATOGRAPHY FLOW POLYMERIZATION PURIFICATION DEPENDENCE DILUTE |
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Description |
The use of large DNAs in preparing multivalent vaccines that will eventually give protective immunity against multiple pathogenic microbes is becoming a major debate nowadays. One of the important issues in ensuring the successful implementation of the new vaccine technology is the development of a chromatographic technique that can handle larger DNAs. This paper reports the development of a novel conical monolithic column format with pore and surface characteristics engineered for the isolation of 25 kbp DNA in a single step fashion. An effective method of eliminating wall channelling, a defect of most conventional monolithic chromatography systems which has caused significant loss of product, was applied to maximise DNA recovery. This method was based on a systematic reduction of wall channel size based on a predetermined correlation between column's back pressure and wall channel size of a particular monolith pore size. (C) 2013 Elsevier B.V. All rights reserved.
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Publisher |
ELSEVIER SCIENCE BV
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Date |
2014-10-15T14:00:43Z
2014-10-15T14:00:43Z 2013 |
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Type |
Article
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Identifier |
JOURNAL OF CHROMATOGRAPHY A, 1318156-162
0021-9673 1873-3778 http://dx.doi.org/10.1016/j.chroma.2013.10.011 http://dspace.library.iitb.ac.in/jspui/handle/100/15049 |
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Language |
en
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