Assessment of the metabolic capacity and adaptability of aromatic hydrocarbon degrading strain Pseudomonas putida CSV86 in aerobic chemostat culture
DSpace at IIT Bombay
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Title |
Assessment of the metabolic capacity and adaptability of aromatic hydrocarbon degrading strain Pseudomonas putida CSV86 in aerobic chemostat culture
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Creator |
NIGAM, A
PHALE, PS WANGIKAR, PP |
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Subject |
Pseudomonas putida CSV86
Benzyl alcohol Dilution rate Oxygen uptake rate Chemostat CATABOLITE REPRESSION CONTROL INTERMEDIATE DEGRADATION PATHWAY PROTEIN ACID INHIBITION SUBSTRATE TOLUENE PHENOL |
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Description |
Pseudomonas putida CSV86 utilizes aromatic compounds preferentially over sugars and co-metabolizes aromatics along with organic acids. In the present study, the metabolic capacity and adaptability of strain CSV86 were assessed in a chemostat at benzyl alcohol concentrations ranging from 1 g l(-1) to 3 g l(-1) and in the presence of glucose and succinate by systematically varying the dilution rate. Complete removal of benzyl alcohol was achieved for loadings up to 640 mg l(-1) h(-1) in presence of benzyl alcohol alone. The strain responded within 1 min towards step changes in substrate loading as indicated by an increase in the oxygen uptake rate, presumably as a result of excess metabolic capacity. These results suggest that CSV86 exhibits considerable metabolic elasticity upon increase in substrate load. Metabolic elasticity of the microorganism is an important parameter in wastewater treatment plants due to the changing substrate loads. (C) 2012 Elsevier Ltd. All rights reserved.
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Publisher |
ELSEVIER SCI LTD
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Date |
2014-10-15T14:08:53Z
2014-10-15T14:08:53Z 2012 |
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Type |
Article
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Identifier |
BIORESOURCE TECHNOLOGY, 114484-491
http://dx.doi.org/10.1016/j.biortech.2012.03.007 http://dspace.library.iitb.ac.in/jspui/handle/100/15065 |
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Language |
en
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