Record Details

Assessment of the metabolic capacity and adaptability of aromatic hydrocarbon degrading strain Pseudomonas putida CSV86 in aerobic chemostat culture

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Assessment of the metabolic capacity and adaptability of aromatic hydrocarbon degrading strain Pseudomonas putida CSV86 in aerobic chemostat culture
 
Creator NIGAM, A
PHALE, PS
WANGIKAR, PP
 
Subject Pseudomonas putida CSV86
Benzyl alcohol
Dilution rate
Oxygen uptake rate
Chemostat
CATABOLITE REPRESSION CONTROL
INTERMEDIATE
DEGRADATION
PATHWAY
PROTEIN
ACID
INHIBITION
SUBSTRATE
TOLUENE
PHENOL
 
Description Pseudomonas putida CSV86 utilizes aromatic compounds preferentially over sugars and co-metabolizes aromatics along with organic acids. In the present study, the metabolic capacity and adaptability of strain CSV86 were assessed in a chemostat at benzyl alcohol concentrations ranging from 1 g l(-1) to 3 g l(-1) and in the presence of glucose and succinate by systematically varying the dilution rate. Complete removal of benzyl alcohol was achieved for loadings up to 640 mg l(-1) h(-1) in presence of benzyl alcohol alone. The strain responded within 1 min towards step changes in substrate loading as indicated by an increase in the oxygen uptake rate, presumably as a result of excess metabolic capacity. These results suggest that CSV86 exhibits considerable metabolic elasticity upon increase in substrate load. Metabolic elasticity of the microorganism is an important parameter in wastewater treatment plants due to the changing substrate loads. (C) 2012 Elsevier Ltd. All rights reserved.
 
Publisher ELSEVIER SCI LTD
 
Date 2014-10-15T14:08:53Z
2014-10-15T14:08:53Z
2012
 
Type Article
 
Identifier BIORESOURCE TECHNOLOGY, 114484-491
http://dx.doi.org/10.1016/j.biortech.2012.03.007
http://dspace.library.iitb.ac.in/jspui/handle/100/15065
 
Language en