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Incorporation of 4 '-C-aminomethyl-2 '-O-methylthymidine into DNA by thermophilic DNA polymerases

DSpace at IIT Bombay

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Title Incorporation of 4 '-C-aminomethyl-2 '-O-methylthymidine into DNA by thermophilic DNA polymerases
 
Creator NAWALE, GN
GORE, KR
HOBARTNER, C
PRADEEPKUMAR, PI
 
Subject ENZYMATIC RECOGNITION
RNA
EVOLUTION
OLIGONUCLEOTIDES
5'-TRIPHOSPHATES
THERAPEUTICS
NUCLEOTIDES
REPERTOIRE
THYMIDINES
APTAMERS
 
Description The dual modified nucleotide 4'-C-aminomethyl-2'-O-methylthymidine 5'-triphosphate was synthesized and enzymatically incorporated into DNA by the thermophilic DNA polymerases Pfu and Therminator III. The dual ribose modification imparted increased exonuclease resistance to DNA compared to the well-known 2'-O-methyl modification.
 
Publisher ROYAL SOC CHEMISTRY
 
Date 2014-10-15T14:12:59Z
2014-10-15T14:12:59Z
2012
 
Type Article
 
Identifier CHEMICAL COMMUNICATIONS, 48(77)9619-9621
http://dx.doi.org/10.1039/c2cc35222b
http://dspace.library.iitb.ac.in/jspui/handle/100/15073
 
Language en