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Surfactant aided biodegradation of NAPLs by Burkholderia multivorans: Comparison between Triton X-100 and rhamnolipid JBR-515

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Title Surfactant aided biodegradation of NAPLs by Burkholderia multivorans: Comparison between Triton X-100 and rhamnolipid JBR-515
 
Creator MOHANTY, S
MUKHERJI, S
 
Subject Triton X-100
Rhamnolipid JBR-515
Cell surface hydrophobicity
Contact angle
Zeta potential
DIESEL OIL BIODEGRADATION
PSEUDOMONAS-AERUGINOSA
NONIONIC SURFACTANTS
EXIGUOBACTERIUM-AURANTIACUM
AROMATIC-HYDROCARBONS
CELL HYDROPHOBICITY
DEGRADATION
BIOSURFACTANT
PHENANTHRENE
BIOAVAILABILITY
 
Description Both chemical surfactants and biosurfactants have been effectively used for the degradation of petroleum hydrocarbons. Chemical surfactants are known to enhance biodegradation effectively while activity of biosurfactants is also comparable and they have the additional advantage of being biodegradable. However, the mode of action of chemical surfactants and biosurfactants may vary. This work was conducted to determine the mode of action of Triton X-100 and rhamnolipid JBR-515 by exploring the factors affecting the process of surfactant aided biodegradation of model non aqueous phase liquids (NAPLs) by a naphthalene degrader, Burkholderia multivorans (NG1). Emulsification studies, growth rate and degradation rate studies were conducted and correlated with alteration in cell surface properties including surface hydrophobicity, cell surface charge and cell surface functional groups. Triton X-100 and JBR-515 demonstrated distinct mode of uptake of NAPLs. Triton X-100 enhanced bioavailability by emulsification and supported direct interfacial uptake of model NAPLs by B. multivorans (NG1). Conversely, the biosurfactant rhamnolipid JBR-515 did not demonstrate emulsification of NAPLs and enhanced bioavailability through micellar solubilization. NAPL composition influenced the alteration in the cell surface properties. For both the surfactants, increase in surfactant concentration increased the rate of utilization of aliphatic hydrocarbons from the NAPLs. (c) 2012 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2014-10-15T15:29:26Z
2014-10-15T15:29:26Z
2013
 
Type Article
 
Identifier COLLOIDS AND SURFACES B-BIOINTERFACES, 102644-652
http://dx.doi.org/10.1016/j.colsurfb.2012.08.064
http://dspace.library.iitb.ac.in/jspui/handle/100/15155
 
Language en