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Temporal sequence and cell cycle cues in the assembly of host factors at the yeast 2 micron plasmid partitioning locus

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Title Temporal sequence and cell cycle cues in the assembly of host factors at the yeast 2 micron plasmid partitioning locus
 
Creator MA, CH
CUI, H
HAJRA, S
ROWLEY, PA
FEKETE, C
SARKESHIK, A
GHOSH, SK
YATES, JR
JAYARAM, M
 
Subject CHROMATIN-REMODELING COMPLEX
2-MU-M CIRCLE PLASMID
SACCHAROMYCES-CEREVISIAE
BUDDING YEAST
CHROMOSOME SEGREGATION
DNA-REPLICATION
S-PHASE
CENP-A
SPINDLE MICROTUBULES
MITOTIC CHROMOSOMES
 
Description The Saccharomyces cerevisiae 2 micron plasmid exemplifies a benign but selfish genome, whose stability approaches that of the chromosomes of its host. The plasmid partitioning locus STB (stability locus) displays certain functional analogies with centromeres along with critical distinctions, a significant one being the absence of the kinetochore complex at STB. The remodels the structure of chromatin (RSC) chromatin remodeling complex, the nuclear motor Kip1, the histone H3 variant Cse4 and the cohesin complex associate with both loci. These factors appear to contribute to plasmid segregation either directly or indirectly through their roles in chromosome segregation. Assembly and disassembly of the plasmid-coded partitioning proteins Rep1 and Rep2 and host factors at STB follow a temporal hierarchy during the cell cycle. Assembly is initiated by STB association of [Rsc8-Rsc58], followed by [Rep1-Rep2-Kip1] and [Cse4-Rsc2-Sth1] recruitment, and culminates in cohesin assembly. Disassembly starts with dissociation of RSC components, is followed by cohesin disassembly and Cse4 exit during anaphase and late telophase, respectively. [Rep1-Rep2-Kip1] persists through G1 of the ensuing cell cycle. The de novo assembly of the 'partitioning complex' is cued by the innate cell cycle clock and is dependent on DNA replication. Shared functional attributes of STB and centromere (CEN) are consistent with a potential evolutionary link between them.
 
Publisher OXFORD UNIV PRESS
 
Date 2014-10-15T17:12:16Z
2014-10-15T17:12:16Z
2013
 
Type Article
 
Identifier NUCLEIC ACIDS RESEARCH, 41(4)2340-2353
http://dx.doi.org/10.1093/nar/gks1338
http://dspace.library.iitb.ac.in/jspui/handle/100/15297
 
Language en