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Design, synthesis, biophysical and primer extension studies of novel acyclic butyl nucleic acid (BuNA)

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Title Design, synthesis, biophysical and primer extension studies of novel acyclic butyl nucleic acid (BuNA)
 
Creator KUMAR, V
GORE, KR
PRADEEPKUMAR, PI
KESAVAN, V
 
Subject THROMBIN BINDING APTAMER
BASE-PAIRING PROPERTIES
I-MOTIF STRUCTURE
HYBRIDIZATION PROPERTIES
NUCLEOSIDE ANALOGS
ANTISENSE OLIGONUCLEOTIDES
CHEMICAL-MODIFICATIONS
ENZYMATIC STABILITY
POLYMERASE CRYSTAL
DUPLEX FORMATION
 
Description A novel nucleic acid analogue called acyclic (S)-butyl nucleic acid (BuNA) composed of an acyclic backbone containing a phosphodiester linkage and bearing natural nucleobases was synthesized. Next, (S)-BuNA nucleotides were incorporated in DNA strands and their effect on duplex stability and changes in structural conformation were investigated. Circular dichroism (CD), UV-melting and non-denatured gel electrophoresis (native PAGE) studies revealed that (S)-BuNA is capable of making duplexes with its complementary strands and integration of (S)-BuNA nucleotides into DNA duplex does not alter the B-type-helical structure of the duplex. Furthermore, (S)-BuNA oligonucleotides and (S)-BuNA substituted DNA strands were studied as primer extensions by DNA polymerases. This study revealed that the acyclic scaffold is tolerated by enzymes and is therefore to some extent biocompatible.
 
Publisher ROYAL SOC CHEMISTRY
 
Date 2014-10-16T05:32:05Z
2014-10-16T05:32:05Z
2013
 
Type Article
 
Identifier ORGANIC & BIOMOLECULAR CHEMISTRY, 11(35)5853-5865
1477-0520
1477-0539
http://dx.doi.org/10.1039/c3ob41244j
http://dspace.library.iitb.ac.in/jspui/handle/100/15344
 
Language en