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Role of extracellular cues to trigger the metabolic phase shifting from acidogenesis to solventogenesis in Clostridium acetobutylicum

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Title Role of extracellular cues to trigger the metabolic phase shifting from acidogenesis to solventogenesis in Clostridium acetobutylicum
 
Creator KUMAR, M
GAYEN, K
SAINI, S
 
Subject Clostridium acetobutylicum
Metabolism
Butanol
SOLVENT PRODUCTION
BUTANOL PRODUCTION
CONTINUOUS-CULTURE
ACETONE
FERMENTATION
SPORULATION
EXPRESSION
LIMITATION
GLUCOSE
BATCH
 
Description Clostridium acetobutylicum exhibits a two-step metabolic pathway where substrates are first converted to organic acids accompanied by a decrease in pH. The acids are then assimilated to organic solvents. The transition from the acid-producing (acidogenesis) to the solvent-producing phase (solventogenesis) is controlled by integration of a number of cellular and environmental cues, whose precise mode of action are not well understood. In this study, a series of batch experiments were performed to understand the impact of extracellular cues in regulating the dynamics of acidogenesis and solventogenesis. It is demonstrated that the two phases operate independently of each other and the growth phase of the cell, i.e. the cues controlling a phase are not linked to the status of the other phase or the growth phase of the cell. Kinetic experiments demonstrated that there exist two previously uncharacterized negative feedback loops controlling the amounts of acids produced in the acidogenesis phase. (C) 2013 Elsevier Ltd. All rights reserved.
 
Publisher ELSEVIER SCI LTD
 
Date 2014-10-16T05:45:07Z
2014-10-16T05:45:07Z
2013
 
Type Article
 
Identifier BIORESOURCE TECHNOLOGY, 13855-62
http://dx.doi.org/10.1016/j.biortech.2013.03.159
http://dspace.library.iitb.ac.in/jspui/handle/100/15370
 
Language en