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In vitro application of paclitaxel loaded magnetoliposomes for combined chemotherapy and hyperthermia

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Title In vitro application of paclitaxel loaded magnetoliposomes for combined chemotherapy and hyperthermia
 
Creator KULSHRESTHA, P
GOGOI, M
BAHADUR, D
BANERJEE, R
 
Subject Thermosensitive magnetoliposomes
Paclitaxel
Hyperthermia
Nanoparticles
Alternating magnetic field
TUMOR-BEARING MICE
THERMOSENSITIVE MAGNETOLIPOSOMES
ELECTROMAGNETIC-FIELD
SOLID TUMORS
LIPOSOMES
RELEASE
DELIVERY
NANOPARTICLES
AGENTS
5-FLUOROURACIL
 
Description Paclitaxel loaded thermosensitive magnetoliposomes containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-glycerol (PG) were prepared by thin film hydration method. Encapsulation efficiencies of paclitaxel and citric acid coated Fe3O4 nanoparticles were 83 +/- 3% and 74.6 +/- 5%, respectively. Based on the release study, DPPC/PG in 9:1 (w/w) liposomes (PCPG) formulation was found to be thermosensitive and showed 46 fold higher drug release at 43 degrees C than at 37 degrees C. Drug release was done under an alternating magnetic field of intensity 10 kA/m and a fixed frequency of 423 kHz. In-vitro cytotoxicity and hyperthermia studies were carried out using a human cervical cancer cell line (HeLa). IC50 value of the magnetoliposomes formulation was 100 nM. When the magnetoliposomes with 100 nM drug was used to treat HeLa cells in combination with hyperthermia under AC magnetic field, 89% cells were killed and were found to be more effective than either hyperthermia or chemotherapy alone. So, PCPG liposomes which co-encapsulate both Fe3O4 nanoparticles and paclitaxel may be useful for combined chemotherapy and hyperthermia. (C) 2012 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2014-10-16T05:52:37Z
2014-10-16T05:52:37Z
2012
 
Type Article
 
Identifier COLLOIDS AND SURFACES B-BIOINTERFACES, 961-7
http://dx.doi.org/10.1016/j.colsurfb.2012.02.029
http://dspace.library.iitb.ac.in/jspui/handle/100/15385
 
Language en