Record Details

Isolation, identification and removal of filamentous organism from SND based SBR degrading nitrophenols

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Isolation, identification and removal of filamentous organism from SND based SBR degrading nitrophenols
 
Creator KULKARNI, PM
 
Subject Nitrophenols
Thiosphaera pantotropha
Heterotrophic nitrification
Aerobic denitrification
SND
SBR
SEQUENCING BATCH REACTOR
DISSOLVED-OXYGEN CONCENTRATION
BIOLOGICAL NUTRIENT REMOVAL
ACTIVATED-SLUDGE BULKING
4-NITROPHENOL BIODEGRADATION
BACTERIAL-POPULATIONS
UASB REACTORS
P-NITROPHENOL
WASTE-WATER
GROWTH
 
Description Four identical lab scale sequencing batch reactors R, R1, R2, and R3, were used to assess nitrophenol biodegradation using a single sludge biomass containing Thiosphaera pantotropha. Nitrophenols [4-Nitrophenol (4-NP), 2,4-dinitrophenol (2,4-DNP) and 2,4,6-trinitrophenol (2,4,6-TNP)] were biotransformed by heterotrophic nitrification and aerobic denitrification (SND). Reactor R was used as background control, whereas R1, R2, and R3 were fed with 4-NP, 2,4-DNP, and 2,4,6-TNP, respectively. The concentration of each nitrophenol was gradually increased from 2.5 to 200 mg/l along with increase in COD, during acclimation studies. The final COD maintained was 4,500 mg/l with each nitrophenolic loading of 200 mg/l. During late phase of acclimation and HRT study, a filamentous organism started appearing in 2,4-DNP and 2,4,6-TNP bioreactors. Filaments were never found in 4-NP and background control reactor. Biochemistry and physiology behind filamentous organism development, was studied to obtain permanent solution for its removal. The effect of different input parameters such as COD loading, DO levels, SVI etc. were analyzed. The morphology and development of filamentous organism were examined extensively using microscopic techniques involving ESEM, oil immersion, phase contrast, and dark field microscopy. The organism was grown and isolated on selective agar plates and was identified as member of Streptomyses species.
 
Publisher SPRINGER
 
Date 2014-10-16T05:56:38Z
2014-10-16T05:56:38Z
2012
 
Type Article
 
Identifier BIODEGRADATION, 23(3)455-463
http://dx.doi.org/10.1007/s10532-011-9524-7
http://dspace.library.iitb.ac.in/jspui/handle/100/15393
 
Language en