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Anomalies in the motion dynamics of long-flagella mutants of Chlamydomonas reinhardtii

DSpace at IIT Bombay

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Title Anomalies in the motion dynamics of long-flagella mutants of Chlamydomonas reinhardtii
 
Creator KHONA, DK
RAO, VG
MOTIWALLA, MJ
VARMA, PCS
KASHYAP, AR
DAS, K
SHIROLIKAR, SM
BORDE, L
DHARMADHIKARI, JA
DHARMADHIKARI, AK
MUKHOPADHYAY, S
MATHUR, D
D'SOUZA, JS
 
Subject Chlamydomonas reinhardtii
Flagella
Motion dynamics
Swimming velocity
Beat frequency
Waveforms
Long-flagella mutants
Motility
Dynein arms
SLIDING FILAMENT MODEL
OUTER DYNEIN ARMS
BEND PROPAGATION
GENETIC-ANALYSIS
LENGTH CONTROL
HEAVY-CHAIN
MICROTUBULES
MOVEMENT
DOUBLET
CELLS
 
Description Chlamydomonas reinhardtii has long been used as a model organism in studies of cell motility and flagellar dynamics. The motility of the well-conserved '9+2' axoneme in its flagella remains a subject of immense curiosity. Using high-speed videography and morphological analyses, we have characterized long-flagella mutants (lf1, lf2-1, lf2-5, lf3-2, and lf4) of C. reinhardtii for biophysical parameters such as swimming velocities, waveforms, beat frequencies, and swimming trajectories. These mutants are aberrant in proteins involved in the regulation of flagellar length and bring about a phenotypic increase in this length. Our results reveal that the flagellar beat frequency and swimming velocity are negatively correlated with the length of the flagella. When compared to the wild-type, any increase in the flagellar length reduces both the swimming velocities (by 26-57%) and beat frequencies (by 8-16%). We demonstrate that with no apparent aberrations/ultrastructural deformities in the mutant axonemes, it is this increased length that has a critical role to play in the motion dynamics of C. reinhardtii cells, and, provided there are no significant changes in their flagellar proteome, any increase in this length compromises the swimming velocity either by reduction of the beat frequency or by an alteration in the waveform of the flagella.
 
Publisher SPRINGER
 
Date 2014-10-16T06:31:38Z
2014-10-16T06:31:38Z
2013
 
Type Article
 
Identifier JOURNAL OF BIOLOGICAL PHYSICS, 39(1)1-14
http://dx.doi.org/10.1007/s10867-012-9282-8
http://dspace.library.iitb.ac.in/jspui/handle/100/15440
 
Language en