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Improved functionalization of electrospun PLLA/gelatin scaffold by alternate soaking method for bone tissue engineering

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Title Improved functionalization of electrospun PLLA/gelatin scaffold by alternate soaking method for bone tissue engineering
 
Creator JAISWAL, AK
KADAM, SS
SONI, VP
BELLARE, JR
 
Subject Biomaterials
Biomimetic
Electrospinning
Mineralization
Cell proliferation
Complement activation
ALCOHOL) HYDROGEL MATRICES
GLUTARALDEHYDE CROSS-LINKING
IN-VITRO
OSTEOGENIC DIFFERENTIATION
HYDROXYAPATITE DEPOSITION
GELATIN SCAFFOLDS
APATITE FORMATION
STEM-CELLS
FABRICATION
MINERALIZATION
 
Description Biomimetic biomaterials are widely being explored as scaffold for bone regeneration. In this study, we prepared poly-l-lactic acid/hydroxyapatite (PLLA/HA) and poly-L-lactic acid/gelatin/hydroxyapatite (PLLA/Gel/HA) scaffold by electrospinning of poly-L-lactic acid (PLLA) and a blend of poly-l-lactic acid/gelatin (PLLA/Gel) followed by hydroxyapatite (HA) mineralization via alternate soaking in calcium and phosphate (Ca-P) solutions. HA growth on scaffold after each soaking cycle was confirmed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The functional groups (COO and -NH2) of gelatin in the PLLA/Gel scaffold facilitated the surface nucleation of HA as compared to the PLLA scaffold. Leaching study showed HA in PLLA/Gel/HA scaffold acts as binder of gelatin and eliminates use of toxic crosslinking agents. In vitro cell attachment on these scaffolds was assessed by using human osteosarcoma cells (MG-63). Cell proliferation on scaffolds was examined by MTT assay. MTT results clearly indicated that mineralized scaffolds did not inhibit the eventual cell proliferation. Alkaline phosphatase (ALP) activity of MG-63 cells was found to be the highest on PLLA/Gel/HA at day 7 compared to all other scaffolds. Complement activation study revealed minimum terminal complement complex (TCC) concentration for PLLA/Gel and PLLA/Gel/HA (617.33 and 654.13 ng/mL respectively). These results demonstrate the proficiency of PLLA/Gel/HA scaffold in better osteostimulation with lesser immune response, which attributed to synergistic role of gelatin and HA. Thus, by mimicking the natural microenvironment PLLA/Gel/HA scaffolds can become the choice of material in bone tissue engineering. (C) 2013 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2014-10-16T12:42:07Z
2014-10-16T12:42:07Z
2013
 
Type Article
 
Identifier APPLIED SURFACE SCIENCE, 268477-488
http://dx.doi.org/10.1016/j.apsusc.2012.12.152
http://dspace.library.iitb.ac.in/jspui/handle/100/15574
 
Language en