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Bone Healing Evaluation of Nanofibrous Composite Scaffolds in Rat Calvarial Defects: A Comparative Study

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Title Bone Healing Evaluation of Nanofibrous Composite Scaffolds in Rat Calvarial Defects: A Comparative Study
 
Creator JAISWAL, AK
DHUMAL, RV
GHOSH, S
CHAUDHARI, P
NEMANI, H
SONI, VP
VANAGE, GR
BELLARE, JR
 
Subject Scaffold
Electrospinning
In Vivo Implantation
Biomaterial
Micro-CT
TISSUE-ENGINEERING SCAFFOLDS
MESENCHYMAL STEM-CELLS
CRITICAL-SIZE DEFECT
BIODEGRADABLE COLLOIDAL GELS
BETA-TRICALCIUM PHOSPHATE
ALTERNATE SOAKING PROCESS
IN-VIVO
HYDROXYAPATITE CERAMICS
POLYMERIC NANOFIBERS
REGENERATION
 
Description We studied the in vivo performance of scaffolds consisting of nanofibrous poly(L-lactic acid) (P) and blend of poly(L-lactic acid/gelatin) (PG) prepared by electrospinning and further composited them with hydroxyapatite (HA) via alternate soaking method, to get poly(L-lactic acid)/hydroxyapatite (PH) and poly(L-lactic acid)/gelatin/hydroxyapatite (PGH) scaffolds respectively. The purpose of this study was to assess and compare bone regeneration potential of electrospun P, PG and electrospun-alternate soaked PH and PGH scaffolds using rat as an animal model by creating two 5 mm circular defects in calvaria. The respective scaffolds were implanted into the defects as one side implantation and both side implantation. Defects left empty served as a negative control for one side implantation and as sham control for both side implantations. The outcomes of the scaffold implantation were determined after 6 and 10 weeks by digital radiography, micro-CT, dual-energy X-ray absorptiometry (DEXA) and histological analysis. PGH scaffold regenerated maximum amount of new bone with high bone mineral density (BMD) into the defects and complete closure occurred in just 6 weeks while other scaffolds failed to close the defects completely. PGH group exhibited highest BMD value after 10 weeks. Histological findings showed abundant osteoblasts and initiation of matrix mineralization in HA containing scaffolds. Masson's trichrome staining showed collagen deposition in all scaffold groups except sham control group. Biochemical and haematological parameters were well with in normal range, indicating no infection due to scaffold implantation. These results prove PGH scaffold as a potential biomaterial for bone regenerative medicine.
 
Publisher AMER SCIENTIFIC PUBLISHERS
 
Date 2014-10-16T12:42:37Z
2014-10-16T12:42:37Z
2013
 
Type Article
 
Identifier JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 9(12)2073-2085
http://dx.doi.org/10.1166/jbn.2013.1736
http://dspace.library.iitb.ac.in/jspui/handle/100/15575
 
Language en