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Hardystonite improves biocompatibility and strength of electrospun polycaprolactone nanofibers over hydroxyapatite: A comparative study

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Title Hardystonite improves biocompatibility and strength of electrospun polycaprolactone nanofibers over hydroxyapatite: A comparative study
 
Creator JAISWAL, AK
CHHABRA, H
KADAM, SS
LONDHE, K
SONI, VP
BELLARE, JR
 
Subject Hardystonite
Hydroxyapatite
Cell proliferation
Cellular infiltration
Electrospinning
Mineralization
TISSUE ENGINEERING APPLICATIONS
ALKALINE-PHOSPHATASE ACTIVITY
IN-VIVO BEHAVIOR
SUBSTITUTED HYDROXYAPATITE
3-DIMENSIONAL SCAFFOLDS
MECHANICAL-PROPERTIES
COMPOSITE NANOFIBERS
FIBROUS SCAFFOLDS
BONE REGENERATION
ZINC
 
Description The aim of this study was to compare physico-chemical and biological properties of hydroxyapatite (HA) and hardystonite (HS) based composite scaffolds. Hardystonite (Ca2ZnSi2O7) powders were synthesized by a sol-gel method while polycaprolactone-hardystonite (PCL-HS) and polycaprolactone-hydroxyapatite (PCL-HA) were fabricated in nanofibrous form by electrospinning. The physico-chemical and biological properties such as tensile strength, cell proliferation, cell infiltration and alkaline phosphatase activity were determined on both kinds of scaffolds. We found that PCL-HS scaffolds had better mechanical strength compared to PCL-HA scaffolds. Addition of HA and HS particles to PCL did not show any inhibitory effect on blood biocompatibility of scaffolds when assessed by hemolysis assay. The in vitro cellular behavior was evaluated by growing murine adipose-tissue-derived stem cells (mE-ASCs) over the scaffolds. Enhanced cell proliferation and improved cellular infiltrations on PCL-HS scaffolds were observed when compared to HA containing scaffolds. PCL-HS scaffolds exhibited a significant increase in alkaline phosphatase (ALP) activity and better mineralization of the matrix in comparison to PCL-HA scaffolds. These results clearly demonstrate the stimulatory role of Zn and Si present in HS based composite scaffolds, suggesting their potential application for bone tissue engineering. (C) 2013 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2014-10-16T12:43:37Z
2014-10-16T12:43:37Z
2013
 
Type Article
 
Identifier MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 33(5)2926-2936
http://dx.doi.org/10.1016/j.msec.2013.03.020
http://dspace.library.iitb.ac.in/jspui/handle/100/15577
 
Language en