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Mineralization of nanohydroxyapatite on electrospun poly(L-lactic acid)/gelatin by an alternate soaking process: A biomimetic scaffold for bone regeneration

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Title Mineralization of nanohydroxyapatite on electrospun poly(L-lactic acid)/gelatin by an alternate soaking process: A biomimetic scaffold for bone regeneration
 
Creator JAISWAL, AK
CHANDRA, V
BHONDE, RR
SONI, VP
BELLARE, JR
 
Subject Biocompatibility
biomaterials
biomimetic
electrospinning
mineralization
poly(L-lactic acid/gelatin)
nanohydroxyapatite
stem cell differentiation
adipose stem cells
MESENCHYMAL STEM-CELLS
MARROW STROMAL CELLS
OSTEOGENIC DIFFERENTIATION
NANOCOMPOSITE SCAFFOLDS
COMPOSITE SCAFFOLDS
IN-VITRO
TISSUE REGENERATION
COLLAGEN
HYDROXYAPATITE
FABRICATION
 
Description Biomimetic nanocomposite scaffolds were fabricated by electrospinning poly(l-lactic acid) and a blend of poly(L-lactic acid)/gelatin to eliminate the use of collagen. The scaffolds were mineralized via alternate soaking in calcium and phosphate solutions, whereby 66.8% nanohydroxyapatite formation was successfully induced which is similar to that of native human bone (60%). The poly(L-lactic acid)/gelatin scaffolds had uniform nanohydroxyapatite formation throughout the scaffold. The mineralization enhanced the tensile modulus and tensile strength without increasing the brittleness. The in vitro biocompatibility of scaffolds was evaluated with murine adipose tissue-derived stem cells. The scaffolds with nanohydroxyapatite aided cell attachment and promoted cell-cell interaction. The mineralization and osteocalcin expression of the murine adipose tissue-derived stem cells were maximum in the poly(L-lactic acid)/gelatin/nanohydroxyapatite scaffold. Therefore, the gelatin and nanohydroxyapatite in poly(L-lactic acid)/gelatin/nanohydroxyapatite scaffolds provided cues for the differentiation of murine adipose tissue-derived stem cells. The biochemical nature of poly(L-lactic acid)/gelatin/nanohydroxyapatite scaffold accelerated osteogenic differentiation and could be a potential candidate for bone regeneration.
 
Publisher SAGE PUBLICATIONS LTD
 
Date 2014-10-16T12:44:07Z
2014-10-16T12:44:07Z
2012
 
Type Article
 
Identifier JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, 27(4)356-374
http://dx.doi.org/10.1177/0883911512447211
http://dspace.library.iitb.ac.in/jspui/handle/100/15578
 
Language en