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Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents

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Title Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents
 
Creator GHOSH, S
PATIL, S
AHIRE, M
KITTURE, R
KALE, S
PARDESI, K
CAMEOTRA, SS
BELLARE, J
DHAVALE, DD
JABGUNDE, A
CHOPADE, BA
 
Subject Dioscorea bulbifera tuber extract
silver nanoparticles
antimicrobial synergy
L. VAR SATIVA
ACINETOBACTER-BAUMANNII
SPP. TUBERS
CLERODANE DITERPENOIDS
ANTIBACTERIAL ACTIVITY
STAPHYLOCOCCUS-AUREUS
ANTIOXIDANT ACTIVITY
BIOLOGICAL SYNTHESIS
METAL NANOPARTICLES
BAFOUDIOSBULBIN-B
 
Description Background: Development of an environmentally benign process for the synthesis of silver nanomaterials is an important aspect of current nanotechnology research. Among the 600-species of the genus Dioscorea, Dioscorea bulbifera has profound therapeutic applications due to its unique phytochemistry. In this paper, we report on the rapid synthesis of silver nanoparticles by reduction of aqueous Ag+ ions using D. bulbifera tuber extract. Methods and results: Phytochemical analysis revealed that D. bulbifera tuber extract is rich in flavonoid, phenolics, reducing sugars, starch, diosgenin, ascorbic acid, and citric acid. The biosynthesis process was quite fast, and silver nanoparticles were formed within 5 hours. Ultraviolet-visible absorption spectroscopy, transmission electron microscopy, high-resolution transmission electron microscopy, energy dispersive spectroscopy, and x-ray diffraction confirmed reduction of the Ag+ ions. Varied morphology of the bioreduced silver nanoparticles included spheres, triangles, and hexagons. Optimization studies revealed that the maximum rate of synthesis could be achieved with 0.7 mM AgNO3 solution at 50 degrees C in 5 hours. The resulting silver nanoparticles were found to possess potent antibacterial activity against both Gram-negative and Gram-positive bacteria. Beta-lactam (piperacillin) and macrolide (erythromycin) antibiotics showed a 3.6-fold and 3-fold increase, respectively, in combination with silver nanoparticles selectively against multidrug-resistant Acinetobacter baumannii. Notable synergy was seen between silver nanoparticles and chloramphenicol or vancomycin against Pseudomonas aeruginosa, and was supported by a 4.9-fold and 4.2-fold increase in zone diameter, respectively. Similarly, we found a maximum 11.8-fold increase in zone diameter of streptomycin when combined with silver nanoparticles against E. coli, providing strong evidence for the synergistic action of a combination of antibiotics and silver nanoparticles. Conclusion: This is the first report on the synthesis of silver nanoparticles using D. bulbifera tuber extract followed by an estimation of its synergistic potential for enhancement of the antibacterial activity of broad spectrum antimicrobial agents.
 
Publisher DOVE MEDICAL PRESS LTD
 
Date 2014-10-16T13:34:03Z
2014-10-16T13:34:03Z
2012
 
Type Article
 
Identifier INTERNATIONAL JOURNAL OF NANOMEDICINE, 7483-496
http://dx.doi.org/10.2147/IJN.S24793
http://dspace.library.iitb.ac.in/jspui/handle/100/15677
 
Language en