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A nanoparticulate injectable hydrogel as a tissue engineering scaffold for multiple growth factor delivery for bone regeneration

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Title A nanoparticulate injectable hydrogel as a tissue engineering scaffold for multiple growth factor delivery for bone regeneration
 
Creator DYONDI, D
WEBSTER, TJ
BANERJEE, R
 
Subject bone tissue engineering
bone morphogenetic protein 7 (BMP7)
basic fibroblast growth factor (bFGF)
hydrogel
nanoparticles
osteoblasts
MESENCHYMAL STEM-CELLS
DIFFERENTIATION IN-VITRO
GELLAN GUM
OSTEOBLAST DIFFERENTIATION
MORPHOGENETIC PROTEINS
OXIDE NANOPARTICLES
CHONDROGENESIS
MINERALIZATION
PROLIFERATION
SYSTEM
 
Description Gellan xanthan gels have been shown to be excellent carriers for growth factors and as matrices for several tissue engineering applications. Gellan xanthan gels along with chitosan nanoparticles of 297 +/- 61 nm diameter, basic fibroblast growth factor (bFGF), and bone morphogenetic protein 7 (BMP7) were employed in a dual growth factor delivery system to promote the differentiation of human fetal osteoblasts. An injectable system with ionic and temperature gelation was optimized and characterized. The nanoparticle loaded gels showed significantly improved cell proliferation and differentiation due to the sustained release of growth factors. A differentiation marker study was conducted, analyzed, and compared to understand the effect of single vs dual growth factors and free vs encapsulated growth factors. Dual growth factor loaded gels showed a higher alkaline phosphatase and calcium deposition compared to single growth factor loaded gels. The results suggest that encapsulation and stabilization of growth factors within nanoparticles and gels are promising for bone regeneration. Gellan xanthan gels also showed antibacterial effects against Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis, the common pathogens in implant failure.
 
Publisher DOVE MEDICAL PRESS LTD
 
Date 2014-10-16T14:13:26Z
2014-10-16T14:13:26Z
2013
 
Type Article
 
Identifier INTERNATIONAL JOURNAL OF NANOMEDICINE, 847-59
http://dx.doi.org/10.2147/IJN.S37953
http://dspace.library.iitb.ac.in/jspui/handle/100/15755
 
Language en