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Development and Characterization of Dual Growth Factor Loaded In Situ Gelling Biopolymeric System for Tissue Engineering Applications

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Title Development and Characterization of Dual Growth Factor Loaded In Situ Gelling Biopolymeric System for Tissue Engineering Applications
 
Creator DYONDI, D
CHANDRA, V
BHONDE, RR
BANERJEE, R
 
Subject Tissue Engineering
Hydrogels
Adipose Tissue Derived Stem Cells
Chitosan Nanoparticles
Dual Release
GELLAN GUM
ARTICULAR-CARTILAGE
HYDROGELS
DELIVERY
GELS
 
Description In the past few decades, use of biodegradable scaffolds for tissue regeneration has emerged as a promising therapeutic approach with considerable success at clinical levels. The following study describes development of one such system an in situ gelling minimally invasive system using bacterial polysaccharides-Gellan and Xanthan as scaffolds for various tissue engineering applications. Gelation time studies for Gellan: Xanthan hydrogels was carried out to determine the most suitable ratio of the two gel components such that the gel mixture should remain a low viscosity fluid at room temperature but should form a highly viscous gel upon injection at the injury site (i.e., at body temperature) within a few minutes. The porous microstructure of the gels was observed by scanning electron micrographs (SEM) and transmission electron microscopy (TEM). A dual growth factor release system with platelet derived growth factors (PDGF-BB) and basic fibroblast growth factor (bFGF) was developed where growth factors were encapsulated within chitosan nanoparticles embedded in the gels as well as directly within the gel. Adipose tissue derived stem cell (ADSC) differentiation and production of glycosaminoglycans (GAG) was observed within 7 days as was tested by safranin-O staining. MU assay showed >90% viability for both L929 fibroblasts and ADSCs.
 
Publisher AMER SCIENTIFIC PUBLISHERS
 
Date 2014-10-16T14:13:56Z
2014-10-16T14:13:56Z
2012
 
Type Article
 
Identifier JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, 2(1)67-75
http://dx.doi.org/10.1166/jbt.2012.1036
http://dspace.library.iitb.ac.in/jspui/handle/100/15756
 
Language en