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A novel selectable marker based on Aspergillus niger arginase expression

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Title A novel selectable marker based on Aspergillus niger arginase expression
 
Creator DAVE, K
AHUJA, M
JAYASHRI, TN
SIROLA, RB
PUNEKAR, NS
 
Subject Fungal transformation
Arginase (agaA) disruption
Nutritional markers
Aspergillus niger
FILAMENTOUS FUNGI
SACCHAROMYCES-CEREVISIAE
HETEROLOGOUS EXPRESSION
NEUROSPORA-CRASSA
GENE
DISRUPTION
NIDULANS
TRANSFORMATION
REPRESSION
INDUCTION
 
Description Selectable markers are valuable tools in transforming asexual fungi like Aspergillus niger. An arginase (agaA) expression vector and a suitable arginase-disrupted host would define a novel nutritional marker/selection for transformation. The development of such a marker was successfully achieved in two steps. The single genomic copy of A. niger arginase gene was disrupted by homologous integration of the bar marker. The agaA disruptant was subsequently complemented by transforming it with agaA expression vectors. Both citA and trpC promoters were able to drive the expression of arginase cDNA. Such agaA(+) transformants displayed arginase expression pattern distinct from that of the parent strain. The results are also consistent with a single catabolic route for arginine in this fungus. A simple yet novel arginine-based selection for filamentous fungal transformation is thus described. (C) 2012 Elsevier Inc. All rights reserved.
 
Publisher ELSEVIER SCIENCE INC
 
Date 2014-10-16T14:54:55Z
2014-10-16T14:54:55Z
2012
 
Type Article
 
Identifier ENZYME AND MICROBIAL TECHNOLOGY, 51(1)53-58
http://dx.doi.org/10.1016/j.enzmictec.2012.04.001
http://dspace.library.iitb.ac.in/jspui/handle/100/15837
 
Language en