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Sanguinarine suppresses IgE induced inflammatory responses through inhibition of type II PtdIns 47kinase(s)

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Title Sanguinarine suppresses IgE induced inflammatory responses through inhibition of type II PtdIns 47kinase(s)
 
Creator BOJJIREDDY, N
SINHA, RK
PANDA, D
SUBRAHMANYAM, G
 
Subject Phosphatidylinositol 4-kinase
Sanguinarine
beta Hexosaminidase
RBL 2H3 cells
PHOSPHATIDYLINOSITOL 4-KINASES
SPLENIC LYMPHOCYTES
ASSEMBLY DYNAMICS
CANCER-CELLS
ACTIVATION
PHOSPHORYLATION
ALPHA
APOPTOSIS
PATHWAYS
AKT
 
Description The effects of sanguinarine on IgE mediated early signaling mechanisms leading to inflammatory mediators release were investigated. Pretreatment of RBL 2H3 cells with sanguinarine inhibited IgE induced activation of type II PtdIns 4-kinase activity. Concomitant with type II PtdIns 4-kinase inhibition, sanguinarine also inhibited IgE induced degranulation and 0 hexosaminidase release in RBL 2H3 cells. In vitro assays showed sanguinarine inhibited type II Ptdlns 4-kinase activity in a dose dependent fashion with no effect on PtdIns 3-kinase activity. Fluorescence spectroscopic studies suggested that sanguinarine binds to type II PtdIns 4-kinases alpha and beta isoforms with a K-d of 2.4 and 1.8 mu M, respectively. Kinetic studies showed that sanguinarine competes with PtdIns binding site of type II PtdIns 4-kinase beta. These results suggest that the anti-inflammatory effects of sanguinarine on PtdIns 3-kinase signaling pathway are more likely an indirect effect and emphasize the importance of the cross talk between type II Ptdlns 4-kinases and Ptdlns 3-kinases. (C) 2013 Elsevier Inc. All rights reserved.
 
Publisher ELSEVIER SCIENCE INC
 
Date 2014-10-17T04:27:56Z
2014-10-17T04:27:56Z
2013
 
Type Article
 
Identifier ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 537(2)192-197
http://dx.doi.org/10.1016/j.abb.2013.07.017
http://dspace.library.iitb.ac.in/jspui/handle/100/15948
 
Language en