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A way to identify archaellins in Halobacterium salinarum archaella by FLAG-tagging

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Title A way to identify archaellins in Halobacterium salinarum archaella by FLAG-tagging
 
Creator BEZNOSOV, SN
PYATIBRATOV, MG
VELURI, PS
MITRA, S
FEDOROV, OV
 
Subject Haloarchaea
Archaeal flagella
Flagellin
Immuno-electron microscopy
METHANOCOCCUS-VOLTAE
BACTERIAL FLAGELLA
MULTIGENE FAMILY
IV PILI
GENES
IDENTIFICATION
TRANSFORMATION
FILAMENT
PROTEINS
CLEAVAGE
 
Description In the current study, haloarchaea Halobacterium salinarum cells were transformed individually with each of the modified archaellin genes (flaA1, flaA2 and flaB2) containing an oligonucleotide insert encoding the FLAG peptide (DYKDDDDK). The insertion site was selected to expose the FLAG peptide on the archaella filament surface. Three types of transformed cells synthesizing archaella, containing A1, A2, or B2 archaellin modified with FLAG peptide were obtained. Electron microscopy of archaella has demonstrated that in each case the FLAG peptide is available for the specific antibody binding. It was shown for the first time that the B2 archaellin, like archaellins A1 and A2, is found along the whole filament length.
 
Publisher VERSITA
 
Date 2014-10-17T04:50:11Z
2014-10-17T04:50:11Z
2013
 
Type Article
 
Identifier CENTRAL EUROPEAN JOURNAL OF BIOLOGY, 8(9)828-834
http://dx.doi.org/10.2478/s11535-013-0202-0
http://dspace.library.iitb.ac.in/jspui/handle/100/15992
 
Language en