Record Details

Model based optimization of high cell density cultivation of nitrogen-fixing cyanobacteria

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Model based optimization of high cell density cultivation of nitrogen-fixing cyanobacteria
 
Creator ALAGESAN, S
GAUDANA, SB
KRISHNAKUMAR, S
WANGIKAR, PP
 
Subject Mixotrophic
Fed-batch
Cyanothece
Feeding strategy
Photolimitation
DYNAMIC OPTIMIZATION
SPIRULINA-PLATENSIS
ESCHERICHIA-COLI
BATCH CULTURES
GROWTH-RATE
ATCC 51142
LIGHT
CYANOTHECE
PHOTOBIOREACTORS
CARBON
 
Description In the present study, fed-batch cultivation of Cyanothece sp. ATCC 51142, a known hydrogen producer, was optimized for maximizing biomass production. Decline in growth of this organism in dense cultures was attributed to increased substrate consumption for maintenance and respiration, and photolimitation due to self shading. A model incorporating these aspects was developed, and by using control vector parameterization (CVP), substrate feeding recipe was optimized to achieve 12-fold higher biomass concentration. The optimization results were verified experimentally on shake flask and bioreactor. The latter resulted in greater exponential growth rate possibly by overcoming photolimitation by simulating flashing light effect. Such a strategy can be readily applied for mixotrophic cultivation of cyanobacterial cultures in the first stage followed by photoautotrophic growth at the production stage. (C) 2013 Elsevier Ltd. All rights reserved.
 
Publisher ELSEVIER SCI LTD
 
Date 2014-10-17T05:48:37Z
2014-10-17T05:48:37Z
2013
 
Type Article
 
Identifier BIORESOURCE TECHNOLOGY, 148228-233
0960-8524
1873-2976
http://dx.doi.org/10.1016/j.biortech.2013.08.144
http://dspace.library.iitb.ac.in/jspui/handle/100/16107
 
Language en