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Genome Segregation and Packaging Machinery in Acanthamoeba polyphaga Mimivirus Is Reminiscent of Bacterial Apparatus

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Title Genome Segregation and Packaging Machinery in Acanthamoeba polyphaga Mimivirus Is Reminiscent of Bacterial Apparatus
 
Creator CHELIKANI, V
RANJAN, T
ZADE, A
SHUKLA, A
KONDABAGIL, K
 
Subject COLI CHROMOSOME SEGREGATION
SITE-SPECIFIC RECOMBINATION
ESCHERICHIA-COLI
TOPOISOMERASE-IV
DNA TRANSLOCATION
DIMER RESOLUTION
FTSK-TRANSLOCASE
CELL-DIVISION
REPLICATION
MECHANISM
 
Description Genome packaging is a critical step in the virion assembly process. The putative ATP-driven genome packaging motor of Acanthamoeba polyphaga mimivirus (APMV) and other nucleocytoplasmic large DNA viruses (NCLDVs) is a distant ortholog of prokaryotic chromosome segregation motors, such as FtsK and HerA, rather than other viral packaging motors, such as large terminase. Intriguingly, APMV also encodes other components, i.e., three putative serine recombinases and a putative type II topoisomerase, all of which are essential for chromosome segregation in prokaryotes. Based on our analyses of these components and taking the limited available literature into account, here we propose for the first time a model for genome segregation and packaging in APMV that can possibly be extended to NCLDV subfamilies, except perhaps Poxviridae and Ascoviridae. This model might represent a unique variation of the prokaryotic system acquired and contrived by the large DNA viruses of eukaryotes. It is also consistent with previous observations that unicellular eukaryotes, such as amoebae, are melting pots for the advent of chimeric organisms with novel mechanisms.
 
Publisher AMER SOC MICROBIOLOGY
 
Date 2014-12-28T11:16:54Z
2014-12-28T11:16:54Z
2014
 
Type Article
 
Identifier JOURNAL OF VIROLOGY, 88(11)6069-6075
0022-538X
1098-5514
http://dx.doi.org/10.1128/JVI.03199-13
http://dspace.library.iitb.ac.in/jspui/handle/100/16336
 
Language English