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Qualitative SERS analysis of G-quadruplex DNAs using selective stabilising ligands

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Title Qualitative SERS analysis of G-quadruplex DNAs using selective stabilising ligands
 
Creator GRACIE, K
DHAMODHARAN, V
PRADEEPKUMAR, PI
FAULDS, K
GRAHAM, D
 
Subject SURFACE-ENHANCED-RAMAN
TELOMERIC G-QUADRUPLEX
INTRAMOLECULAR G-QUADRUPLEX
TELOMESTATIN
SPECTROSCOPY
SCATTERING
CELLS
BISQUINOLINIUM
RECOGNITION
INHIBITOR
 
Description Nucleic acids are of key biological importance due to their range of functions and ability to form various different structures, with an example of emerging significance being quadruplexes formed by guanine-rich sequences. These guanine rich sequences are found in different regions of the genome such as telomeres, gene promoters and introns and UTRs of mRNAs. Here a new approach has been developed that utilises surface enhanced Raman scattering (SERS) for the detection of the formation of G-quadruplexes. Three G-quadruplex stabilising ligands that each have their own unique SERS response were used in this study and their ability to act as reporters assessed. A SERS response was only obtained from the ligands in the absence of G-quadruplex formation. This resulted in an "on/off" method which was successfully used to qualitatively detect the formation of G-quadruplex using quadruplex-forming sequences such as human telomeric and C-MYC promoter DNAs. The unique SERS spectra of each stabilising ligand offer the potential for use of SERS to study higher order DNA structures. This work shows that the ligands used can act simultaneously as a potential therapeutic stabilising agent and a SERS reporter, therefore allowing the use of SERS as a method of analysis of the formation of G-quadruplex DNAs.
 
Publisher ROYAL SOC CHEMISTRY
 
Date 2014-12-28T11:39:58Z
2014-12-28T11:39:58Z
2014
 
Type Article
 
Identifier ANALYST, 139(18)4458-4465
0003-2654
1364-5528
http://dx.doi.org/10.1039/c4an00551a
http://dspace.library.iitb.ac.in/jspui/handle/100/16382
 
Language English