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Production, characterization, and immunogenicity of a secreted form of Plasmodium falciparum merozoite surface protein 4 produced in Bacillus subtilis

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Title Production, characterization, and immunogenicity of a secreted form of Plasmodium falciparum merozoite surface protein 4 produced in Bacillus subtilis
 
Creator CHITTIBABU, G
MA, C
NETTER, HJ
NORONHA, SB
COPPEL, RL
 
Subject Malaria
Plasmodium falciparum
Bacillus subtilis
PfMSP4
Subunit vaccine
Protein production
ANCHORED MEMBRANE-PROTEINS
LETHAL CHALLENGE
EXPRESSION
IMMUNIZATION
MALARIA
YOELII
MEROZOITE-SURFACE-PROTEIN-4
SPECIFICITY
PROTECTION
ADJUVANTS
 
Description Plasmodium falciparum is the causative agent of the most serious form of malaria. Although a combination of control measures has significantly limited malaria morbidity and mortality in the last few years, it is generally agreed that sustained control or even eradication will require additional tools including an effective malaria vaccine. Merozoite surface protein 4, MSP4, which is present during the asexual stage of P. falciparum, is a recognized target that would be useful in a subunit vaccine against blood stages of malaria. Falciparum malaria is most prevalent in developing countries, and this in turn leads to a requirement for safe, low-cost vaccines. We have attempted to utilize the nonpathogenic, gram-positive organism Bacillus subtilis to produce PfMSP4. PfMSP4 was secreted into the culture medium at a yield of 4.5 mg/L. Characterization studies including SDS-PAGE, mass spectrometry, and N-terminal sequencing indicated that the B. subtilis expression system secreted a full length PfMSP4 protein compared to a truncated version in Escherichia coli. Equivalent amounts of purified B. subtilis and E. coli-derived PfMSP4 were used for immunization studies, resulting in statistically significant higher mean titer values for the B. subtilis-derived immunogen. The mouse antibodies raised against B. subtilis produced PfMSP4 that were reactive to parasite proteins as evidenced by immunoblotting on parasite lysate and indirect immunofluorescence assays of fixed parasites. The B. subtilis expression system, in contrast to E. coli, expresses higher amounts of full length PfMSP4 products, decreased levels of aggregates, and allows the development of simplified downstream processing procedures.
 
Publisher SPRINGER
 
Date 2014-12-28T11:45:59Z
2014-12-28T11:45:59Z
2014
 
Type Article
 
Identifier APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(8)3669-3678
0175-7598
1432-0614
http://dx.doi.org/10.1007/s00253-013-5275-1
http://dspace.library.iitb.ac.in/jspui/handle/100/16394
 
Language English