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Biocompatible Amphiphilic Pentablock Copolymeric Nanoparticles for Anti-Cancer Drug Delivery

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Title Biocompatible Amphiphilic Pentablock Copolymeric Nanoparticles for Anti-Cancer Drug Delivery
 
Creator BYAGARI, K
SHANAVAS, A
RENGAN, AK
KUNDU, GC
SRIVASTAVA, R
 
Subject Pluronic F68
Poly(lactide-co-glycolide)
Amphiphilic Copolymer
Nanoparticles
Vesicles
Dialysis
Emulsion Solvent Evaporation
BLOCK-COPOLYMERS
TRIBLOCK COPOLYMERS
DOCETAXEL
MICELLES
BEHAVIOR
RELEASE
PROTEIN
PLGA
 
Description A pentablock copolymer of Poly(Lactide-co-Glycolide) and Pluronic F68 was synthesized using ring-opening polymerization and characterized by NMR and FTIR for confirming the structure of the block copolymer. TG-DTA studies showed PLGA:Pluronic ratio to be 4:1. As the PLGA-PEO-PPO-PEO-PLGA Pentablock Copolymer (PPPC) prepared is amphiphilic, its Critical Vesicular Concentration, was measured, which was lower at 37 degrees C than at 25 degrees C, which could provide better stability to the system at physiological temperature. The nanoparticles of PPPC vary in topographyand range from 150 to 500 nm in size, according to the synthesis route used viz Emulsion Solvent Evaporation and simple dialysis. Pentablock copolymer nanoparticles were found to entrap about 84% of hydrophobic drug, docetaxel. Drug release profile of docetaxel showed about 50% release in first 2 hours at pH 4.6 and about 80% docetaxel was released at pH 7.4, at the end of 2 days. The PPPC nanoparticles was found to be biocompatible to L929 cell lines up to 1 mg/ml concentration. Preliminary in vitro cytotoxic effect of docetaxel loaded PPPC nanoparticles against four different cancer cell lines showed 50% inhibitory concentration of 6 nM in A431 (Squamous cell carcinoma), 250 nM in HeLa (Cervical carcinoma), 800 nM in PC3 (Prostate carcinoma) and 1 mu M in KB (Epidermoid carcinoma) cells.
 
Publisher AMER SCIENTIFIC PUBLISHERS
 
Date 2014-12-28T14:08:47Z
2014-12-28T14:08:47Z
2014
 
Type Article
 
Identifier JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 10(1)109-119
1550-7033
1550-7041
http://dx.doi.org/10.1166/jbn.2014.1791
http://dspace.library.iitb.ac.in/jspui/handle/100/16714
 
Language English