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Fyn kinase regulates type II PtdIns 4-kinases in RBL 2H3 cells

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Title Fyn kinase regulates type II PtdIns 4-kinases in RBL 2H3 cells
 
Creator BOJJIREDDY, N
SINHA, RK
SUBRAHMANYAM, G
 
Subject Fc epsilon RI receptor
Phosphatidylinositol 4-phosphate
SH2 domain
beta-Hexosamindase
FC-EPSILON-RI
AFFINITY IGE RECEPTOR
RAT SPLENIC LYMPHOCYTES
SRC FAMILY KINASES
PHOSPHATIDYLINOSITOL 4-KINASE
MAST-CELL
TYROSINE KINASES
KEY COMPONENT
RBL-2H3 CELLS
ACTIVATION
 
Description Type II phosphatidylinositol 4-kinases are implicated in Fc epsilon RI-mediated signaling cascades leading to release of inflammatory molecules. Cross-linking of Fc epsilon RI on RBL 2H3 cells results in protein tyrosine phosphorylation and activation of type II PtdIns 4-kinase activity. Protein tyrosine kinase(s) that phosphorylate type II PtdIns 4-kinase(s) in RBL 2H3 cells remains elusive and is being addressed in this manuscript. Anti-Fyn kinase antibodies co-immunoprecipitated type II PtdIns 4-kinase activity from Fc epsilon RI cross-linked RBL 2H3 cells. In reciprocal assays, His-tagged types II PtdIns 4-kinases were shown to pull down Fyn kinase. Further, anti-Fyn immunoprecipitates were shown to phosphorylate type II PtdIns 4-kinase alpha and beta in in vitro assays. Pull down studies with GST-Fyn-SH2 and GST-Fyn-SH3 domains showed that type II PtdIns 4-kinases associate with Fyn-SH2 domain. Knockdown of Fyn kinase in RBL 2H3 cells abrogated activation of type II PtdIns 4-kinase activity in response to Fc epsilon RI cross-linking and type II PtdIns 4-kinase activity in anti-phosphotyrosine immunoprecipitates. Knockdown of Fyn kinase was also strongly correlated with a reduction in beta-hexosaminidase release in response to Fc epsilon RI cross-linking. These results suggest that type II PtdIns 4-kinases act downstream of Fyn kinase in Fc epsilon RI signaling cascades and are regulated by Fyn kinase.
 
Publisher SPRINGER
 
Date 2014-12-28T14:38:21Z
2014-12-28T14:38:21Z
2014
 
Type Article
 
Identifier MOLECULAR AND CELLULAR BIOCHEMISTRY, 387(1/2)63-70
0300-8177
1573-4919
http://dx.doi.org/10.1007/s11010-013-1871-8
http://dspace.library.iitb.ac.in/jspui/handle/100/16773
 
Language English