Fyn kinase regulates type II PtdIns 4-kinases in RBL 2H3 cells
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Title |
Fyn kinase regulates type II PtdIns 4-kinases in RBL 2H3 cells
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Creator |
BOJJIREDDY, N
SINHA, RK SUBRAHMANYAM, G |
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Subject |
Fc epsilon RI receptor
Phosphatidylinositol 4-phosphate SH2 domain beta-Hexosamindase FC-EPSILON-RI AFFINITY IGE RECEPTOR RAT SPLENIC LYMPHOCYTES SRC FAMILY KINASES PHOSPHATIDYLINOSITOL 4-KINASE MAST-CELL TYROSINE KINASES KEY COMPONENT RBL-2H3 CELLS ACTIVATION |
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Description |
Type II phosphatidylinositol 4-kinases are implicated in Fc epsilon RI-mediated signaling cascades leading to release of inflammatory molecules. Cross-linking of Fc epsilon RI on RBL 2H3 cells results in protein tyrosine phosphorylation and activation of type II PtdIns 4-kinase activity. Protein tyrosine kinase(s) that phosphorylate type II PtdIns 4-kinase(s) in RBL 2H3 cells remains elusive and is being addressed in this manuscript. Anti-Fyn kinase antibodies co-immunoprecipitated type II PtdIns 4-kinase activity from Fc epsilon RI cross-linked RBL 2H3 cells. In reciprocal assays, His-tagged types II PtdIns 4-kinases were shown to pull down Fyn kinase. Further, anti-Fyn immunoprecipitates were shown to phosphorylate type II PtdIns 4-kinase alpha and beta in in vitro assays. Pull down studies with GST-Fyn-SH2 and GST-Fyn-SH3 domains showed that type II PtdIns 4-kinases associate with Fyn-SH2 domain. Knockdown of Fyn kinase in RBL 2H3 cells abrogated activation of type II PtdIns 4-kinase activity in response to Fc epsilon RI cross-linking and type II PtdIns 4-kinase activity in anti-phosphotyrosine immunoprecipitates. Knockdown of Fyn kinase was also strongly correlated with a reduction in beta-hexosaminidase release in response to Fc epsilon RI cross-linking. These results suggest that type II PtdIns 4-kinases act downstream of Fyn kinase in Fc epsilon RI signaling cascades and are regulated by Fyn kinase.
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Publisher |
SPRINGER
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Date |
2014-12-28T14:38:21Z
2014-12-28T14:38:21Z 2014 |
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Type |
Article
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Identifier |
MOLECULAR AND CELLULAR BIOCHEMISTRY, 387(1/2)63-70
0300-8177 1573-4919 http://dx.doi.org/10.1007/s11010-013-1871-8 http://dspace.library.iitb.ac.in/jspui/handle/100/16773 |
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Language |
English
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