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Type II phosphatidylinositol 4-kinases interact with Fc epsilon RI gamma subunit in RBL-2H3 cells

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Title Type II phosphatidylinositol 4-kinases interact with Fc epsilon RI gamma subunit in RBL-2H3 cells
 
Creator BOJJIREDDY, N
SINHA, RK
SUBRAHMANYAM, G
 
Subject Type II PtdIns 4-kinase
RBL-2H3 cells
FceRI gamma
Mast cells
AFFINITY IGE RECEPTOR
TYROSINE KINASE SYK
RBL 2H3 CELLS
SPLENIC LYMPHOCYTES
ZETA-CHAIN
ACTIVATION
ALPHA
PHOSPHORYLATION
DEGRANULATION
 
Description Ligation of high-affinity IgE receptor I (Fc epsilon RI) on RBL-2H3 cells leads to recruitment of Fc epsilon RI and type II phosphatidylinositol 4-kinases (PtdIns 4-kinases) into lipid rafts. Lipid raft integrity is required for the activation of type II PtdIns 4-kinases and signal transduction through Fc epsilon RI gamma during RBL-2H3 cell activation. However, the molecular mechanism by which PtdIns 4-kinases are coupled to Fc epsilon RI signaling is elusive. Here, we report association of type II PtdIns 4-kinase activity with Fc epsilon RI gamma subunit in anti-Fc epsilon RI gamma immunoprecipitates. Fc epsilon RI gamma-associated PtdIns 4-kinase activity increases threefold upon Fc epsilon RI ligation in anti-Fc epsilon RI gamma immunoprecipitates. Biochemical characterization of PtdIns 4-kinase activity associated with Fc epsilon RI gamma reveals that it is a type II PtdIns 4-kinases. Canonical tyrosine residues mutation in Fc epsilon RI gamma ITAM (Y65 and Y76) reveals that these two tyrosine residues in gamma subunit are required for its interaction with type II PtdIns 4-kinases.
 
Publisher SPRINGER
 
Date 2014-12-28T14:38:51Z
2014-12-28T14:38:51Z
2014
 
Type Article
 
Identifier MOLECULAR AND CELLULAR BIOCHEMISTRY, 390(1/2)197-203
0300-8177
1573-4919
http://dx.doi.org/10.1007/s11010-014-1970-1
http://dspace.library.iitb.ac.in/jspui/handle/100/16774
 
Language English