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Piperine inhibits type II phosphatidylinositol 4-kinases: a key component in phosphoinositides turnover

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Title Piperine inhibits type II phosphatidylinositol 4-kinases: a key component in phosphoinositides turnover
 
Creator BOJJIREDDY, N
SINHA, RK
SUBRAHMANYAM, G
 
Subject Type II PtdIns 4-kinase
RBL-2H3 cells
beta-Hexosaminidase
Mast cell
Piperine
INDUCED INFLAMMATORY RESPONSES
RBL 2H3 CELLS
SPLENIC LYMPHOCYTES
RBL-2H3 CELLS
ZETA-CHAIN
RECEPTOR
ACTIVATION
ALPHA
PHOSPHORYLATION
METABOLISM
 
Description Piperine has been shown to have anti-inflammatory properties. The molecular mechanisms by which it mediates anti-inflammatory activities remain elusive. Type II phosphatidylinositol 4-kinase(s) are key components in Fc epsilon RI receptor-mediated signaling leading to inflammatory mediators release in RBL-2H3 cells. The effects of piperine on IgE-mediated signaling and mast cell degranulation were investigated. Pretreatment of RBL-2H3 cells with piperine inhibited IgE-induced activation of type II PtdIns 4-kinase(s). In vitro lipid kinase assays showed piperine-inhibited type II PtdIns 4-kinase activity in a dose-dependent fashion with no effect on PtdIns 3-kinase activity. Concomitantly, pretreatment of RBL-2H3 cells with piperine also inhibited IgE-induced beta-hexosaminidase release in RBL-2H3 cells. These results suggest that type II PtdIns 4-kinases are part of piperine-mediated anti-inflammatory signaling mechanisms.
 
Publisher SPRINGER
 
Date 2014-12-28T14:39:21Z
2014-12-28T14:39:21Z
2014
 
Type Article
 
Identifier MOLECULAR AND CELLULAR BIOCHEMISTRY, 393(1/2)9-15
0300-8177
1573-4919
http://dx.doi.org/10.1007/s11010-014-2041-3
http://dspace.library.iitb.ac.in/jspui/handle/100/16775
 
Language English