Type II PtdIns 4-kinase beta associates with CD4-p56lck complex and is involved in CD4 receptor signaling
DSpace at IIT Bombay
View Archive InfoField | Value | |
Title |
Type II PtdIns 4-kinase beta associates with CD4-p56lck complex and is involved in CD4 receptor signaling
|
|
Creator |
SINHA, RK
BOJJIREDDY, N KANOJIA, D SUBRAHMANYAM, G |
|
Subject |
CD4 receptor
PtdIns 4-kinases T cell activation T-CELL-ACTIVATION RAT SPLENIC LYMPHOCYTES PHOSPHATIDYLINOSITOL 4-KINASES TYROSYL PHOSPHORYLATION ANTIGEN RECEPTOR PROTEIN-TYROSINE KEY COMPONENT CROSS-LINKING ZETA-CHAIN MECHANISMS |
|
Description |
Type II phosphatidylinositol (PtdIns) 4-kinases are involved in the synthesis of PtdIns 4-phosphates and modulate various cell functions like, intracellular signaling, cytoskeletal rearrangements, vesicular trafficking, and pathogen invasion. In CD3 receptor activated T cells, a type II PtdIns 4-kinase beta is recruited to CD3 receptor zeta and plays a role in intracellular calcium release and probably in actin cytoskeleton reorganization. T cell receptor mediated activation is supported by CD4 receptor. The role of type II PtdIns 4-kinase beta in CD4 receptor-mediated signaling was addressed in the present manuscript. Crosslinking of CD4 receptors with monoclonal antibodies showed an increase in CD4-associated PtdIns 4-kinase activity and requires p56(lck) activity. Biochemical characterization suggests that it belongs to type II PtdIns 4-kinase family. shRNA mediated knockdown of type II PtdIns 4-kinase beta showed abrogation of CD4 receptor induced intracellular calcium release. These results suggest that type II PtdIns 4-kinase beta plays an integral part in CD4 receptor-mediated signaling.
|
|
Publisher |
SPRINGER
|
|
Date |
2014-12-28T14:39:51Z
2014-12-28T14:39:51Z 2014 |
|
Type |
Article
|
|
Identifier |
MOLECULAR AND CELLULAR BIOCHEMISTRY, 395(1/2)231-239
0300-8177 1573-4919 http://dx.doi.org/10.1007/s11010-014-2129-9 http://dspace.library.iitb.ac.in/jspui/handle/100/16776 |
|
Language |
English
|
|