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Type II PtdIns 4-kinase beta associates with CD4-p56lck complex and is involved in CD4 receptor signaling

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Title Type II PtdIns 4-kinase beta associates with CD4-p56lck complex and is involved in CD4 receptor signaling
 
Creator SINHA, RK
BOJJIREDDY, N
KANOJIA, D
SUBRAHMANYAM, G
 
Subject CD4 receptor
PtdIns 4-kinases
T cell activation
T-CELL-ACTIVATION
RAT SPLENIC LYMPHOCYTES
PHOSPHATIDYLINOSITOL 4-KINASES
TYROSYL PHOSPHORYLATION
ANTIGEN RECEPTOR
PROTEIN-TYROSINE
KEY COMPONENT
CROSS-LINKING
ZETA-CHAIN
MECHANISMS
 
Description Type II phosphatidylinositol (PtdIns) 4-kinases are involved in the synthesis of PtdIns 4-phosphates and modulate various cell functions like, intracellular signaling, cytoskeletal rearrangements, vesicular trafficking, and pathogen invasion. In CD3 receptor activated T cells, a type II PtdIns 4-kinase beta is recruited to CD3 receptor zeta and plays a role in intracellular calcium release and probably in actin cytoskeleton reorganization. T cell receptor mediated activation is supported by CD4 receptor. The role of type II PtdIns 4-kinase beta in CD4 receptor-mediated signaling was addressed in the present manuscript. Crosslinking of CD4 receptors with monoclonal antibodies showed an increase in CD4-associated PtdIns 4-kinase activity and requires p56(lck) activity. Biochemical characterization suggests that it belongs to type II PtdIns 4-kinase family. shRNA mediated knockdown of type II PtdIns 4-kinase beta showed abrogation of CD4 receptor induced intracellular calcium release. These results suggest that type II PtdIns 4-kinase beta plays an integral part in CD4 receptor-mediated signaling.
 
Publisher SPRINGER
 
Date 2014-12-28T14:39:51Z
2014-12-28T14:39:51Z
2014
 
Type Article
 
Identifier MOLECULAR AND CELLULAR BIOCHEMISTRY, 395(1/2)231-239
0300-8177
1573-4919
http://dx.doi.org/10.1007/s11010-014-2129-9
http://dspace.library.iitb.ac.in/jspui/handle/100/16776
 
Language English