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Dynamics of Gal80p in the Gal80p-Gal3p complex differ significantly from the dynamics in the Gal80p-Gal1p complex: implications for the higher specificity of Gal3p

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Title Dynamics of Gal80p in the Gal80p-Gal3p complex differ significantly from the dynamics in the Gal80p-Gal1p complex: implications for the higher specificity of Gal3p
 
Creator UPADHYAY, SK
 
Subject POISSON-BOLTZMANN EQUATION
SACCHAROMYCES-CEREVISIAE
GENETIC SWITCH
TRANSCRIPTIONAL ACTIVATION
MOLECULAR SIMULATION
PROTEIN INTERFACES
GALACTOSE
YEAST
GALACTOKINASE
GAL1P
 
Description The expression of the GAL gene in Sacharomyces cerevisiae is regulated by three proteins; Gal3p/Gal1p, Gal80p and Gal4p. Both Gal3p and Gal1p act as transcriptional inducers, though Gal3p has a higher activity than Gal1p. The difference in activity may depend on the strength of the interaction and dynamical behavior of these proteins during complex formation with the repressor protein Gal80p. To address these queries we have modeled the binding interface of the Gal1p-Gal80p and Gal3p-Gal80p complexes. The comparison of the dynamics of these proteins in the complex and in the Apo protein was carried out. It was observed that the binding of Gal3p with Gal80p induces significant flexibility in Gal80p on a surface different from the one involved in binding with Gal3p. Several other differences at the interface between the Gal3p-Gal80p and the Gal1p-Gal80p complex were observed, which might permit Gal3p to act as a transcriptional inducer with higher activity. Further, we have discussed the dynamical event and plausible mechanism of complex formation of Gal3p and Gal1p with Gal80p at the molecular level.
 
Publisher ROYAL SOC CHEMISTRY
 
Date 2014-12-28T14:54:23Z
2014-12-28T14:54:23Z
2014
 
Type Article
 
Identifier MOLECULAR BIOSYSTEMS, 10(12)3120-3129
1742-206X
1742-2051
http://dx.doi.org/10.1039/c4mb00371c
http://dspace.library.iitb.ac.in/jspui/handle/100/16805
 
Language English