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Glucose oxidase enzyme immobilized porous silica for improved performance of a glucose biosensor

DSpace at IIT Bombay

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Title Glucose oxidase enzyme immobilized porous silica for improved performance of a glucose biosensor
 
Creator KHAN, AY
NORONHA, SB
BANDYOPADHYAYA, R
 
Subject Biocatalysis
Biosensors
Enzyme activity
Immobilized enzymes
Mesoporous silica
Glucose oxidase
MESOPOROUS MOLECULAR-SIEVES
SBA-15
ENCAPSULATION
MICROSPHERES
ADSORPTION
RESOLUTION
PARTICLES
LYSOZYME
SURFACES
SIZE
 
Description High activity of glucose oxidase (GOD) enzyme (immobilized in porous silica particles) is desirable for a better glucose biosensor. In this work, effect of pore diameter of two porous hosts on enzyme immobilization, activity and glucose sensing was compared. The hosts were amine functionalized: (i) microporous silica (NH2-MS) and (ii) mesoporous silica (NH2-SBA-15). Based on whether the dimension of GOD is either larger or smaller than the pore diameter, GOD was immobilized on either external or internal surface of NH2-MS and NH2-SBA-15, with loadings of 512.5 and 634 mg/g, respectively. However, GOD in NH2-SBA-15 gave a higher normalized absolute activity (NAA), which led to an amperometric sensor with a larger linear range of 0.4-13.0 mM glucose. In comparison, GOD in NH2-MS had a lower NAA and a smaller linear range of 0.4-3.1 mM. In fact, the present GOD-NH2-SBA-15 electrode based sensor was better than other MS and SBA-15 based electrodes reported in literature. Thus, achieving only a high GOD loading (as in NH2-MS) does not necessarily give a good sensor performance. Instead, a host with a relatively larger pore than enzyme, together with optimized electrode composition ensures the sensor to be functional in both hyper- and hypoglycemic range. (C) 2014 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2014-12-28T16:43:51Z
2014-12-28T16:43:51Z
2014
 
Type Article
 
Identifier BIOCHEMICAL ENGINEERING JOURNAL, 9178-85
1369-703X
1873-295X
http://dx.doi.org/10.1016/j.bej.2014.07.011
http://dspace.library.iitb.ac.in/jspui/handle/100/16915
 
Language English