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Protein stabilization and counteraction of denaturing effect of urea by glycine betaine

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Title Protein stabilization and counteraction of denaturing effect of urea by glycine betaine
 
Creator KUMAR, N
KISHORE, N
 
Subject S-peptide
Hydrogen bonding
Molecular dynamics simulation
Denaturation
Radial distribution function
Osmolyte
TRIMETHYLAMINE-N-OXIDE
MOLECULAR-DYNAMICS SIMULATIONS
AQUEOUS UREA
CRATEROSTIGMA-PLANTAGINEUM
INDUCED DENATURATION
MIXED-SOLVENTS
AMINO-ACIDS
S-PEPTIDE
SOLVATION
WATER
 
Description The counteraction of the denaturing effect of urea by osmolytes has been one of the most studied problems of osmolyte action. However, the possibility of synergy in osmolyte mixtures has often been neglected. Here, we report synergy in the glycine betaine (GB)-urea mixture by using a model peptide. The results show that in the GB-urea mixture, GB acts as a stronger osmolyte and urea becomes a weaker denaturing agent. This is reflected by an increase in the exclusion of GB from the peptide surface and a decrease in interactions between the peptide and urea. The cause of this synergistic behaviour includes direct interactions between GB and urea through hydrogen bonding, van der Waals interactions between them and strengthening of hydrogen bonding network of water in the GB-urea mixture. The results obtained in this study provide insights into osmolyte induced counteraction of the denaturing effect of urea. (C) 2014 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2014-12-28T16:57:54Z
2014-12-28T16:57:54Z
2014
 
Type Article
 
Identifier BIOPHYSICAL CHEMISTRY, 18916-24
0301-4622
1873-4200
http://dx.doi.org/10.1016/j.bpc.2014.03.001
http://dspace.library.iitb.ac.in/jspui/handle/100/16943
 
Language English