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pH- and thermosensitive thin lipid layer coated mesoporous magnetic nanoassemblies as a dual drug delivery system towards thermochemotherapy of cancer

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Title pH- and thermosensitive thin lipid layer coated mesoporous magnetic nanoassemblies as a dual drug delivery system towards thermochemotherapy of cancer
 
Creator PRADHAN, L
SRIVASTAVA, R
BAHADUR, D
 
Subject Mesoporous
Nanoassemblies
Dual drug
Lipids
Thermochemotherapy
EVERY 2 WEEKS
IRON-OXIDE
THERAPEUTIC APPLICATIONS
SILICA NANOPARTICLES
CONTROLLED-RELEASE
PACLITAXEL
LIPOSOMES
DOXORUBICIN
CELLS
HYPERTHERMIA
 
Description A new pH-sensitive and thermosensitive dual drug delivery system consisting of thin lipid layer encapsulated mesoporous magnetite nanoassemblies (MMNA) has been developed which can deliver two anticancer drugs simultaneously. The formulation of lipid layer used is 5:2:2:2 w/w, DPPC: cholesterol:DSPE-PEG(2000):MMNA. The structure, morphology and magnetic properties of MMNA and lipid coated MMNA (LMMNA) were thoroughly characterized. This hybrid system was investigated for its ability to carry two anticancer drugs as well as its ability to provide heat under an alternating current magnetic field (ACMF). A very high loading efficiency of up to similar to 81% of doxorubicin hydrochloride (DOX) with an similar to 0.02 mg mg(-1) loading capacity and similar to 60% of paclitaxel (TXL) with an similar to 0.03 mg mg(-1) loading capacity are obtained with LMMNA. A sustained release of drug is observed over a period of 172 h, with better release, of similar to 88:53% (DOX:TXL), at pH 4.3 compared to the similar to 28:26% (DOX:TXL) in physiological conditions (pH 7.4). An enhanced release of similar to 72 and similar to 68% is recorded for DOX and TXL, respectively, during the first hour with the application of an ACMF (similar to 43 degrees C). A greater in vitro cytotoxic effect is observed with the two drugs compared to them individually in HeLa, MCF-7 and HepG2 cancer cells. With the application of an ACMF for 10 min, the cell killing efficiency is improved substantially due to simultaneous thermo- and chemotherapy. Confocal microscopy confirms the internalization of drug loaded MMNA and LMMNA by cells and their morphological changes during thermochemotherapy. (C) 2014 Acts Materialia Inc. Published by Elsevier Ltd. All rights reserved.
 
Publisher ELSEVIER SCI LTD
 
Date 2014-12-28T17:33:54Z
2014-12-28T17:33:54Z
2014
 
Type Article
 
Identifier ACTA BIOMATERIALIA, 10(7)2976-2987
1742-7061
1878-7568
http://dx.doi.org/10.1016/j.actbio.2014.04.011
http://dspace.library.iitb.ac.in/jspui/handle/100/17000
 
Language English