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Self-crosslinked oxidized alginate/gelatin hydrogel as injectable, adhesive biomimetic scaffolds for cartilage regeneration

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Title Self-crosslinked oxidized alginate/gelatin hydrogel as injectable, adhesive biomimetic scaffolds for cartilage regeneration
 
Creator BALAKRISHNAN, B
JOSHI, N
JAYAKRISHNAN, A
BANERJEE, R
 
Subject Hydrogels
Alginate
Injectable
Cartilage
Tissue engineering
B-11 NMR
IN-VITRO
INFLAMMATORY RESPONSES
STROMAL CELLS
DIETARY BORON
STEM-CELLS
COLLAGEN
GELATIN
CHONDROCYTES
CULTURE
 
Description Biopolymeric hydrogels that mimic the properties of extracellular matrix have great potential in promoting cellular migration and proliferation for tissue regeneration. The authors reported earlier that rapidly gelling, biodegradable, injectable hydrogels can be prepared by self-crosslinking of periodate oxidized alginate and gelatin in the presence of borax, without using any toxic crosslinking agents. The present paper investigates the suitability of this hydrogel as a minimally invasive injectable, cell-attractive and adhesive scaffold for cartilage tissue engineering for the treatment of osteoarthritis. Time and frequency sweep rheology analysis confirmed gel formation within 20 s. The hydrogel integrated well with the cartilage tissue, with a burst pressure of 70 +/- 3 mmHg, indicating its adhesive nature. Hydrogel induced negligible inflammatory and oxidative stress responses, a prerequisite for the management and treatment of osteoarthritis. Scanning electron microscopy images of primary murine chondrocytes encapsulated within the matrix revealed attachment of cells onto the hydrogel matrix. Chondrocytes demonstrated viability, proliferation and migration within the matrix, while maintaining their phenotype, as seen by expression of collagen type II and aggrecan, and functionality, as seen by enhanced glycosoaminoglycan (GAG) deposition with time. DNA content and GAG deposition of chondrocytes within the matrix can be tuned by incorporation of bioactive signaling molecules such as dexamethasone, chondroitin sulphate, platelet derived growth factor (PDGF-BB) and combination of these three agents. The results suggest that self-crosslinked oxidized alginate/gelatin hydrogel may be a promising injectable, cell-attracting adhesive matrix for neo-cartilage formation in the management and treatment of osteoarthritis. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
 
Publisher ELSEVIER SCI LTD
 
Date 2014-12-28T17:34:24Z
2014-12-28T17:34:24Z
2014
 
Type Article
 
Identifier ACTA BIOMATERIALIA, 10(8)3650-3663
1742-7061
1878-7568
http://dx.doi.org/10.1016/j.actbio.2014.04.031
http://dspace.library.iitb.ac.in/jspui/handle/100/17001
 
Language English