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A dps promoter based expression system for improved solubility of expressed proteins in Escherichia coli

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Title A dps promoter based expression system for improved solubility of expressed proteins in Escherichia coli
 
Creator SETHIA, PP
RAO, KK
NORONHA, SB
 
Subject Escherichia coli
recombinant protein expression
dps promoter
protein solubility
SOLUBLE RECOMBINANT PROTEINS
DNA-BINDING PROTEIN
HETEROLOGOUS GENE
STATIONARY-PHASE
METABOLIC LOAD
RNA-POLYMERASE
CLONED GENES
E.-COLI
GROWTH
CYTOPLASM
 
Description Escherichia coli is widely used for recombinant protein production due to its well established genetic manipulation techniques and cost effectiveness of the associated production processes. Soluble expression of heterologous recombinant proteins constitutes a major problem in the deployment of bacterial expression systems. We have developed a dps promoter based expression system in E. coli for improved solubility of expressed proteins. The resulting expression system was found to be superior to the IPTG inducible T7 promoter based pET expression system for production of soluble beta-galactosidase, tdTomato, and mCherry. The dps promoter based expression system was shown to be functional in most commonly used strains of E. coli without need for prior genetic manipulation of the host genome.
 
Publisher KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
 
Date 2014-12-29T04:41:56Z
2014-12-29T04:41:56Z
2014
 
Type Article
 
Identifier BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 19(5)790-797
1226-8372
1976-3816
http://dx.doi.org/10.1007/s12257-013-0722-5
http://dspace.library.iitb.ac.in/jspui/handle/100/17103
 
Language English