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C-Terminal Region of MAP7 Domain Containing Protein 3 (MAP7D3) Promotes Microtubule Polymerization by Binding at the C-Terminal Tail of Tubulin

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Title C-Terminal Region of MAP7 Domain Containing Protein 3 (MAP7D3) Promotes Microtubule Polymerization by Binding at the C-Terminal Tail of Tubulin
 
Creator YADAV, S
VERMA, PJ
PANDA, D
 
Subject PAIRED HELICAL FILAMENTS
TAU-PROTEIN
EPITHELIAL-CELLS
MITOTIC SPINDLE
REPEAT DOMAIN
DNA-SEQUENCE
IN-VITRO
ENSCONSIN
DYNAMICS
IDENTIFICATION
 
Description MAP7 domain containing protein 3 (MAP7D3), a newly identified microtubule associated protein, has been shown to promote microtubule assembly and stability. Its microtubule binding region has been reported to consist of two coiled coil motifs located at the N-terminus. It possesses a MAP7 domain near the C-terminus and belongs to the microtubule associated protein 7 (MAP7) family. The MAP7 domain of MAP7 protein has been shown to bind to kinesin-1; however, the role of MAP7 domain in MAP7D3 remains unknown. Based on the bioinformatics analysis of MAP7D3, we hypothesized that the MAP7 domain of MAP7D3 may have microtubule binding activity. Indeed, we found that MAP7 domain of MAP7D3 bound to microtubules as well as enhanced the assembly of microtubules in vitro. Interestingly, a longer fragment MDCT that contained the MAP7 domain (MD) with the C-terminal tail (CT) of the protein promoted microtubule polymerization to a greater extent than MD and CT individually. MDCT stabilized microtubules against dilution induced disassembly. MDCT bound to reconstituted microtubules with an apparent dissociation constant of 3.0 +/- 0.5 mu M. An immunostaining experiment showed that MDCT localized along the length of the preassembled microtubules. Competition experiments with tau indicated that MDCT shares its binding site on microtubules with tau. Further, we present evidence indicating that MDCT binds to the C-terminal tail of tubulin. In addition, MDCT could bind to tubulin in HeLa cell extract. Here, we report a microtubule binding region in the C-terminal region of MAP7D3 that may have a role in regulating microtubule assembly dynamics.
 
Publisher PUBLIC LIBRARY SCIENCE
 
Date 2014-12-29T06:29:53Z
2014-12-29T06:29:53Z
2014
 
Type Article
 
Identifier PLOS ONE, 9(6)
1932-6203
http://dx.doi.org/10.1371/journal.pone.0099539
http://dspace.library.iitb.ac.in/jspui/handle/100/17313
 
Language English