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Flow Characterization of a Spinner Flask for Induced Pluripotent Stem Cell Culture Application

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Title Flow Characterization of a Spinner Flask for Induced Pluripotent Stem Cell Culture Application
 
Creator ISMADI, MZ
GUPTA, P
FOURAS, A
VERMA, P
JADHAV, S
BELLARE, J
HOURIGAN, K
 
Subject LARGE-SCALE EXPANSION
SUSPENSION-CULTURE
BIOREACTOR
MICROCARRIER
BONE
DIFFERENTIATION
CARDIOMYOCYTES
HYDRODYNAMICS
MICROGRAVITY
CULTIVATION
 
Description We present detailed quantitative measurement analyses for flow in a spinner flask with spinning rates between 20 to 45 RPM, utilizing the optical velocimetry measurement technique of Particle Image Velocimetry (PIV). A partial section of the impeller was immersed in the working fluid to reduce the shear forces induced on the cells cultured on microcarriers. Higher rotational speeds improved the mixing effect in the medium at the expense of a higher shear environment. It was found that the mouse induced pluripotent stem (iPS) cells achieved the optimum number of cells over 7 days in 25 RPM suspension culture. This condition translates to 0.0984 Pa of maximum shear stress caused by the interaction of the fluid flow with the bottom surface. However, inverse cell growth was obtained at 28 RPM culture condition. Such a narrow margin demonstrated that mouse iPS cells cultured on microcarriers are very sensitive to mechanical forces. This study provides insight to biomechanical parameters, specifically the shear stress distribution, for a commercially available spinner flask over a wide range of Reynolds number.
 
Publisher PUBLIC LIBRARY SCIENCE
 
Date 2014-12-29T06:32:04Z
2014-12-29T06:32:04Z
2014
 
Type Article
 
Identifier PLOS ONE, 9(10)
1932-6203
http://dx.doi.org/10.1371/journal.pone.0106493
http://dspace.library.iitb.ac.in/jspui/handle/100/17317
 
Language English