Record Details

Cellular internalization and detailed toxicity analysis of protein-immobilized iron oxide nanoparticles

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Cellular internalization and detailed toxicity analysis of protein-immobilized iron oxide nanoparticles
 
Creator SANGANERIA, P
SACHAR, S
CHANDRA, S
BAHADUR, D
RAY, P
KHANNA, A
 
Subject IN-VITRO
SUPERPARAMAGNETIC NANOPARTICLES
MAGNETIC NANOPARTICLES
SURFACE MODIFICATION
DEPENDENT TOXICITY
PARTICLES
STRESS
nanomaterials
nanophase
protein adsorption
cell culture
surface characterization
FTIR
 
Description Iron oxide nanoparticles (IONPs) have been extensively used for biomedical applications like in the diagnosis and treatment of various diseases, as contrast agents in magnetic resonance imaging, and in targeted drug delivery. Despite several attempts, there is a dearth of information with respect to the cellular response and in-depth toxicity analysis of the nanoparticles. Considering the potential benefits of IONPs, there is a need to study the potential cellular damage associated with IONPs. The size and surface of the particles are some critical factors that should be analyzed when evaluating cytotoxicity. Therefore, in this study, we synthesized and characterized bare (7-9 nm) and protein-coated IONPs of diameter 50-70 nm, and evaluated their toxicity on membrane integrity, intracellular accumulation of reactive oxygen species, and mitochondrial activity in mouse fibroblast cell line by lactate dehydrogenase, 2,7-dichlorofluorescein diacetate, and [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) assays, respectively. Our extensive cytotoxicity analysis demonstrated that the size of the IONPs and their surface coating are the critical determinants of cellular response and potential mechanism toward cytotoxicity. The study of the interactions and assessment of potential toxicity of the nanoparticles with cells/tissues is a key determinant when considering their translation in biomedical applications. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 125-134, 2015.
 
Publisher WILEY-BLACKWELL
 
Date 2016-01-14T10:41:38Z
2016-01-14T10:41:38Z
2015
 
Type Article
 
Identifier JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 103(1)125-134
1552-4973
1552-4981
http://dx.doi.org/10.1002/jbm.b.33178
http://dspace.library.iitb.ac.in/jspui/handle/100/17385
 
Language en