Record Details

(R)-PAC Biosynthesis in [BMIM][PF6]/Aqueous Biphasic System Using Saccharomyces cerevisiae BY4741 Cells

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title (R)-PAC Biosynthesis in [BMIM][PF6]/Aqueous Biphasic System Using Saccharomyces cerevisiae BY4741 Cells
 
Creator KANDAR, S
SURESH, AK
NORONHA, SB
 
Subject L-PHENYLACETYL CARBINOL
IMMOBILIZED YEAST-CELLS
IONIC LIQUIDS
CANDIDA-UTILIS
(R)-PHENYLACETYLCARBINOL PRODUCTION
ASYMMETRIC REDUCTION
ESCHERICHIA-COLI
2-PHASE SYSTEMS
BIOTRANSFORMATION
BENZALDEHYDE
(R)-phenylacetylcarbinol
(1R, 2S) ephedrine and (1S, 2S) pseudoephedrine
1-Butyl-3-methylimidazolium hexafluorophosphate
Box-Behnken statistical design
 
Description (R)-phenylacetylcarbinol or (R)-PAC is a pharmaceutical precursor of (1R, 2S) ephedrine and (1S, 2S) pseudoephedrine. Biotransformation of benzaldehyde and glucose by pyruvate decarboxylase produces (R)-PAC. This biotransformation suffers from toxicity of the substrate, product [(R)-PAC] and by-product (benzyl alcohol). In the present study, ionic liquid/aqueous biphasic system was employed to enhance (R)-PAC production. Fermented broth was the reaction medium in which Saccharomyces cerevisiae BY4741 was the source of pyruvate decarboxylase. Hydrophobic ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF6]) was the non-aqueous phase in which toxic compounds reside. Biocompatibility of [BMIM][PF6] and adequate distribution coefficients of benzaldehyde, (R)-PAC and benzyl alcohol were determined. A Box-Behnken design and response surface methodology were used for the optimization of biotransformation variables in order to maximize (R)-PAC yield and productivity. The results showed higher (R)-PAC yield and productivity of similar to 1.5-fold each in the biphasic biotransformation of phase volume ratio 0.05 as compared to the monophasic (conventional) biotransformation. Moreover, the level of major by-product benzyl alcohol was also 3.5-fold lower in biphasic biotransformation. [BMIM][PF6]/aqueous biphasic system is a new approach which could intensify the (R)PAC production.
 
Publisher HUMANA PRESS INC
 
Date 2016-01-14T12:29:11Z
2016-01-14T12:29:11Z
2015
 
Type Article
 
Identifier APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 175(4)1771-1788
0273-2289
1559-0291
http://dx.doi.org/10.1007/s12010-014-1394-0
http://dspace.library.iitb.ac.in/jspui/handle/100/17499
 
Language en