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Coumarine-imino-C2-glucosyl conjugate as receptor for Cu2+ in blood serum milieu, on silica gel sheet and in Hep G2 cells and the characterization of the species of recognition

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Title Coumarine-imino-C2-glucosyl conjugate as receptor for Cu2+ in blood serum milieu, on silica gel sheet and in Hep G2 cells and the characterization of the species of recognition
 
Creator ARETI, S
KHEDKAR, JK
BANDARU, S
TEOTIA, R
BELLARE, J
RAO, CP
 
Subject AMYOTROPHIC-LATERAL-SCLEROSIS
RATIOMETRIC FLUORESCENT
COLORIMETRIC SENSORS
LIVING CELLS
COPPER
ION
CHEMOSENSOR
CU(II)
CHEMODOSIMETER
DERIVATIVES
Coumarine-imino-C2-glucosyl conjugate
Selective recognition of Cu2+
Silica gel solid support
Blood serum milieu
Switch-off fluorescence
 
Description A coumarine-imino-C2-glucosyl conjugate (L) was synthesized and characterized. The conjugate L is found to recognize Cu2+ in aqueous HEPES buffer by exhibiting a 95% fluorescence quenching in pH range 7-10 even in the presence of several biologically and ecologically relevant metal ions. Fluorescence on-off behavior has been clearly demonstrated on the basis of the binding variability of Cu2+ to L. The binding has been elicited through the changes observed in fluorescence, absorption, ESI-MS and H-1 NMR titrations. All the other thirteen metal ions studied did not show any change in the fluorescence emission. These ions do not interfere with the recognition of Cu2+ by L. The structural features of [CuL](2) complex in both the isomeric forms were established by DFT computational calculations. The utility of L has been demonstrated by showing its sensitivity toward Cu2+ on a thin layer of silica gel. The L gives sensitive fluorescence signals for Cu2+ even in blood serum and exhibits appropriate fluorescence responses in living cells. (C) 2015 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2016-01-14T12:51:38Z
2016-01-14T12:51:38Z
2015
 
Type Article
 
Identifier ANALYTICA CHIMICA ACTA, 873,80-87
0003-2670
1873-4324
http://dx.doi.org/10.1016/j.aca.2015.02.065
http://dspace.library.iitb.ac.in/jspui/handle/100/17543
 
Language en