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Self healing hydrogels composed of amyloid nano fibrils for cell culture and stem cell differentiation

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Title Self healing hydrogels composed of amyloid nano fibrils for cell culture and stem cell differentiation
 
Creator JACOB, RS
GHOSH, D
SINGH, PK
BASU, SK
JHA, NN
DAS, S
SUKUL, PK
PATIL, S
SATHAYE, S
KUMAR, A
CHOWDHURY, A
MALIK, S
SEN, S
MAJI, SK
 
Subject ASSEMBLED PEPTIDE NANOTUBES
BETA-PROTEIN
ALZHEIMERS-DISEASE
FMOC-DIPHENYLALANINE
ALPHA-SYNUCLEIN
BIOSENSOR APPLICATIONS
NANOFIBER SCAFFOLD
DESIGN
TISSUE
AGGREGATION
Amyloid hydrogel
Nanofibrils
Self-assembly
Stem cell
Tissue engineering
 
Description Amyloids are highly ordered protein/peptide aggregates associated with human diseases as well as various native biological functions. Given the diverse range of physiochemical properties of amyloids, we hypothesized that higher order amyloid self-assembly could be used for fabricating novel hydrogels for biomaterial applications. For proof of concept, we designed a series of peptides based on the high aggregation prone C-terminus of A beta 42, which is associated with Alzheimer's disease. These Fmoc protected peptides self assemble to beta sheet rich nanofibrils, forming hydrogels that are thermoreversible, non-toxic and thixotropic. Mechanistic studies indicate that while hydrophobic, pi-pi interactions and hydrogen bonding drive amyloid network formation to form supramolecular gel structure, the exposed hydrophobic surface of amyloid fibrils may render thixotropicity to these gels. We have demonstrated the utility of these hydrogels in supporting cell attachment and spreading across a diverse range of cell types. Finally, by tuning the stiffness of these gels through modulation of peptide concentration and salt concentration these hydrogels could be used as scaffolds that can drive differentiation of mesenchymal stem cells. Taken together, our results indicate that small size, ease of custom synthesis, thixotropic nature makes these amyloid-based hydrogels ideally suited for biomaterial/nanotechnology applications. (C) 2015 Elsevier Ltd. All rights reserved.
 
Publisher ELSEVIER SCI LTD
 
Date 2016-01-14T13:05:21Z
2016-01-14T13:05:21Z
2015
 
Type Article
 
Identifier BIOMATERIALS, 54,97-105
0142-9612
1878-5905
http://dx.doi.org/10.1016/j.biomaterials.2015.03.002
http://dspace.library.iitb.ac.in/jspui/handle/100/17570
 
Language en