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Influence of mixotrophic growth on rhythmic oscillations in expression of metabolic pathways in diazotrophic cyanobacterium Cyanothece sp ATCC 51142

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Title Influence of mixotrophic growth on rhythmic oscillations in expression of metabolic pathways in diazotrophic cyanobacterium Cyanothece sp ATCC 51142
 
Creator KRISHNAKUMAR, S
GAUDANA, SB
DIGMURTI, MG
VISWANATHAN, GA
CHETTY, M
WANGIKAR, PP
 
Subject NITROGEN-FIXING CYANOBACTERIA
SP STRAIN ATCC-51142
CONTINUOUS-LIGHT
CONCENTRATING MECHANISM
HYDROGEN-PRODUCTION
GENUS CYANOTHECE
SYNECHOCYSTIS
FIXATION
CARBON
IDENTIFICATION
Transcriptomic analysis
Nitrogen fixation
Hydrogen production
Metabolic oscillations
Cyanobacteria
 
Description This study investigates the influence of mixotrophy on physiology and metabolism by analysis of global gene expression in unicellular diazotrophic cyanobacterium Cyanothece sp. ATCC 51142 ( henceforth Cyanothece 51142). It was found that Cyanothece 51142 continues to oscillate between photosynthesis and respiration in continuous light under mixotrophy with cycle time of similar to 13 h. Mixotrophy is marked by an extended respiratory phase compared with photoautotrophy. It can be argued that glycerol provides supplementary energy for nitrogen fixation, which is derived primarily from the glycogen reserves during photoautotrophy. The genes of NDH complex, cytochrome c oxidase and ATP synthase are significantly overexpressed in mixotrophy during the day compared to autotrophy with synchronous expression of the bidirectional hydrogenase genes possibly to maintain redox balance. However, nitrogenase complex remains exclusive to nighttime metabolism concomitantly with uptake hydrogenase. This study throws light on interrelations between metabolic pathways with implications in design of hydrogen producer strains. (C) 2015 Elsevier Ltd. All rights reserved.
 
Publisher ELSEVIER SCI LTD
 
Date 2016-01-14T13:06:52Z
2016-01-14T13:06:52Z
2015
 
Type Article
 
Identifier BIORESOURCE TECHNOLOGY, 188,145-152
0960-8524
1873-2976
http://dx.doi.org/10.1016/j.biortech.2015.02.016
http://dspace.library.iitb.ac.in/jspui/handle/100/17573
 
Language en