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Biomolecular interactions control the shape of stains from drying droplets of complex fluids

DSpace at IIT Bombay

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Title Biomolecular interactions control the shape of stains from drying droplets of complex fluids
 
Creator HURTH, C
BHARDWAJ, R
ANDALIB, S
FRANKIEWICZ, C
DOBOS, A
ATTINGER, D
ZENHAUSERN, F
 
Subject ATOMIC-FORCE MICROSCOPY
BIOTIN INTERACTIONS
SESSILE DROPLET
EVAPORATION
ADHESION
SUBSTRATE
WATER
Biotin-streptavidin
Microdroplets
Polystyrene beads
Biosensors
Drying mechanism
 
Description When a sessile droplet of a complex fluid dries, a stain forms on the solid surface. The structure and pattern of the stain can be used to detect the presence of a specific chemical compound in the sessile droplet. In the present work, we investigate what parameters of the stain or its formation can be used to characterize the specific interaction between an aqueous dispersion of beads and its receptor immobilized on the surface. We use the biotin-streptavidin system as an experimental model. Clear dissimilarities were observed in the drying sequences on streptavidin-coated substrates of droplets of aqueous solutions containing biotin-coated or streptavidin-coated beads. Fluorescent beads are used in order to visualize the fluid flow field. We show differences in the distribution of the particles on the surface depending on biomolecular interactions between beads and the solid surface. A mechanistic model is proposed to explain the different patterns obtained during drying. The model describes that the beads are left behind the receding wetting line rather than pulled towards the drop center if the biological binding force is comparable to the surface tension of the receding wetting line. Other forces such as the viscous drag, van der Waals forces, and solid-solid friction forces are found negligible. Simple microfluidics experiments are performed to further illustrate the difference in behavior where is adhesion or friction are present between the bead and substrate due to the biological force. The results of the model are in agreement with the experimental observations which provide insight and design capabilities. A better understanding of the effects of the droplet-surface interaction on the drying mechanism is a crucial first step before the identification of drying patterns can be promisingly applied to areas such as immunology and biomarker detection. (C) 2015 Elsevier Ltd. All rights reserved.
 
Publisher PERGAMON-ELSEVIER SCIENCE LTD
 
Date 2016-01-14T13:18:33Z
2016-01-14T13:18:33Z
2015
 
Type Article
 
Identifier CHEMICAL ENGINEERING SCIENCE, 137,398-403
0009-2509
1873-4405
http://dx.doi.org/10.1016/j.ces.2015.06.059
http://dspace.library.iitb.ac.in/jspui/handle/100/17596
 
Language en