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Biodegradation of dimethyl phthalate ester using free cells, entrapped cells of Variovorax sp BS1 and cell free enzyme extracts: A comparative study

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Title Biodegradation of dimethyl phthalate ester using free cells, entrapped cells of Variovorax sp BS1 and cell free enzyme extracts: A comparative study
 
Creator PRASAD, B
SURESH, S
 
Subject ACINETOBACTER-CALCOACETICUS
COMPLETE DEGRADATION
PSEUDOMONAS-CEPACIA
IMMOBILIZED CELLS
CALCIUM ALGINATE
P-CRESOL
DIOXYGENASE
POLYURETHANE
LIGATION
BACTERIA
Alginate
Biodegradation
Cell-free enzymes
Dimethyl phthalate
Variovorax
 
Description Whole cells of Variovorax sp. BS1 had the ability to biodegrade up to 600 mg L-1 (3.08 mM) of dimethyl phthalate (DMP) at the end of 30 h of incubation at 30 degrees C. The total organic carbon analyses suggested mineralization of DMP using isolated bacterial strain. The cell-free extracts of Variovorax sp. BS1 contained mixture of enzymes required for the complete biodegradation of DMP, diethyl phthalate (DEP) and dibutyl phthalate (DBP). Transient intermediates or end products were not detected by HPLC and LC-MS analyses during or after biodegradation of DMP by Variovorax sp. BSI. Addition of Zn+2 to assay mixture containing DMP and cell free extract resulted in the accumulation of detectable quantity of metabolite, ortho-phthalate thereby suggesting that DMP biodegradation proceeded via de-esterification reactions. Cells of Variovorax sp. BSI entrapped in calcium alginate beads could be re-used for atleast ten sequential cycles in plain water with diminutive loss of DMP biodegradation activity. Results suggested that whole cells, alginate entrapped cells of Variovorax sp BS1 and enzymes present in the cell free extracts may be used for efficient biodegradation of dimethyl phthalate. (C) 2014 Elsevier Ltd. All rights reserved.
 
Publisher ELSEVIER SCI LTD
 
Date 2016-01-14T13:49:54Z
2016-01-14T13:49:54Z
2015
 
Type Article
 
Identifier INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 97,179-187
0964-8305
1879-0208
http://dx.doi.org/10.1016/j.ibiod.2014.11.004
http://dspace.library.iitb.ac.in/jspui/handle/100/17657
 
Language en