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Proteomic analysis of Plasmodium falciparum induced alterations in humans from different endemic regions of India to decipher malaria pathogenesis and identify surrogate markers of severity

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Title Proteomic analysis of Plasmodium falciparum induced alterations in humans from different endemic regions of India to decipher malaria pathogenesis and identify surrogate markers of severity
 
Creator RAY, S
KUMAR, V
BHAVE, A
SINGH, V
GOGTAY, NJ
THATTE, UM
TALUKDAR, A
KOCHAR, SK
PATANKAR, S
SRIVASTAVA, S
 
Subject ACUTE-PHASE PROTEINS
COMPLEMENT ACTIVATION
BLOOD-COAGULATION
VIVAX MALARIA
CHILDREN
DISEASE
IDENTIFICATION
INFLAMMATION
MENINGIOMAS
ALPHA
India
Malaria pathogenesis
Plasmodium falciparum
Proteomics
Severe falciparum malaria
Surrogate markers
 
Description India significantly contributes to the global malaria burden and has the largest population in the world at risk of malaria. This study aims to analyze alterations in the human serum proteome as a consequence of non-severe and severe infections by the malaria parasite Plasmodium falciparum to identify markers related to disease severity and to obtain mechanistic insights about disease pathogenesis and host immune responses. In discovery phase of the study, a comprehensive quantitative proteomic analysis was performed using gel-based (2D-DIGE) and gel-free (iTRAQ) techniques on two independent mass spectrometry platforms (ESI-Q-TOF and Q-Exactive mass spectrometry), and selected targets were validated by ELISA. Proteins showing altered serum abundance in falciparum malaria patients revealed the modulation of different physiological pathways including chemokine and cytokine signaling, IL-12 signaling and production in macrophages, complement cascades, blood coagulation, and protein ubiquitination pathways. Some muscle related and cytoskeletal proteins such as titin and galectin-3-binding protein were found to be up-regulated in severe malaria patients. Hemoglobin levels and platelet counts were also found to be drastically lower in severe malaria patients. Identified proteins including serum amyloid A, C-reactive protein, apolipoprotein E and haptoglobin, which exhibited sequential alterations in their serum abundance in different severity levels of malaria, could serve as potential predictive markers for disease severity. To the best of our information, we report here the first comprehensive analysis describing the serum proteomic alterations observed in severe P. falciparum infected patients from different malaria endemic regions of India. This article is part of a Special Issue entitled: Proteomics in India. (C) 2015 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2016-01-15T04:28:38Z
2016-01-15T04:28:38Z
2015
 
Type Article
 
Identifier JOURNAL OF PROTEOMICS, 127(A,SI)103-113
1874-3919
1876-7737
http://dx.doi.org/10.1016/j.jprot.2015.04.032
http://dspace.library.iitb.ac.in/jspui/handle/100/17758
 
Language en